目的:本研究的目的是研究姬松茸乙醇提取物对来曲唑诱导的多囊卵巢综合征(PCOS)大鼠模型的影响。
方法:将30只雌性Wistar大鼠分为5组,每个由六只老鼠组成。阴性对照组施用ImL体积的0.5%羧甲基纤维素(CMC)溶液。将来曲唑(lmg/kg)施用于另外的组,持续21天,以诱导多囊卵巢综合征(PCOS)。在第22天对指定为阳性对照的动物实施安乐死。从第22天至第36天对测试组和标准组进行治疗。实验组以200mg/kg和400mg/kg的剂量口服姬松茸乙醇提取物,而对照组接受1mg/kg剂量的枸橼酸克罗米芬。该研究观察了多囊卵巢疾病患者的各种生理标志物,包括估计的血糖水平,总胆固醇水平,甘油三酯水平,和荷尔蒙波动,如睾丸激素和雌激素水平增加,以及孕酮水平下降。通过检查阴道涂片和卵巢组织病理学变化证实了月经不调的存在。
结果:研究发现,对Agaricussubrufescens的消耗对各种生理参数有重大影响,包括血糖水平,睾酮水平,无排卵,和月经不调。所有治疗干预措施均使血清谷氨酸-草酰乙酸转氨酶(SGOT)和血清谷氨酸-丙酮酸转氨酶(SGPT)的水平显着正常化。来曲唑诱导的多囊卵巢综合征(PCOS)大鼠的尿素和肌酐水平升高。这项研究的结果表明,服用姬松茸对肾功能有保护作用,血清尿素和肌酐水平降低证明了这一点。来曲唑诱导的多囊卵巢综合征(PCOS)大鼠,抑制肝脏合成,促进卵泡不成熟,雄激素分泌的升高导致肝脏和卵巢的重量增加。在所有治疗组中,内分泌器官的重量表现出降低。PCOS标本的组织病理学检查显示囊肿和叶黄素细胞的存在增加。与接受治疗的组相比,未接受治疗的多囊卵巢综合征(PCOS)大鼠组表现出更高的囊肿数量。
结论:本研究证明口服来曲唑导致多囊卵巢疾病的发展。结果表明血糖水平升高,总胆固醇,和甘油三酯,以及激素水平的改变,如睾酮和雌激素增加,孕酮减少。这些荷尔蒙的变化伴随着月经不调,通过阴道涂片检查和卵巢组织病理学分析证实,对照组患有多囊卵巢疾病。接受姬松茸的治疗组表现出血糖下降,总胆固醇,和睾丸激素水平。
OBJECTIVE: The objective of this study is to investigate the effects of an ethanolic extract derived from Agaricus subrufescens on rat models exhibiting Polycystic Ovarian Syndrome (PCOS) induced by Letrozole.
METHODS: A total of thirty female Wistar rats were divided into five groups, each consisting of six rats. The negative control group was administered a volume of 1 mL of a 0.5% solution of carboxy methylcellulose (CMC). Letrozole (1 mg/kg) was administered to additional groups for a duration of 21 days in order to induce polycystic ovary syndrome (PCOS). Animals designated as positive controls were euthanized on the 22nd day. Both the test group and the standard group were subjected to treatment from the 22nd day to the 36th day. The experimental group was administered ethanolic extract of Agaricus subrufescens at doses of 200 mg/kg and 400 mg/kg p.o, while the control group received clomiphene citrate at a dose of 1 mg/kg. The study observed various physiological markers in individuals with polycystic ovarian disease, including estimated blood glucose levels, total cholesterol levels, triglyceride levels, and hormonal fluctuations such as increased testosterone and estrogen levels, as well as decreased progesterone levels. The presence of menstrual irregularities was confirmed through the examination of vaginal smears and histopathological changes in the ovaries.
RESULTS: The consumption of Agaricus subrufescens was found to have a significant impact on various physiological parameters, including blood glucose levels, testosterone levels, anovulation, and menstrual irregularity. All therapeutic interventions significantly normalized the levels of serum glutamic-oxaloacetic transaminase (SGOT) and serum glutamic-pyruvic transaminase (SGPT). The rats with polycystic ovary syndrome (PCOS) that were induced by Letrozole exhibited increased levels of urea and creatinine. The findings of this study indicate that the administration of Agaricus subrufescens therapy has a protective effect on renal function, as evidenced by a reduction in serum levels of urea and creatinine. In rats with polycystic ovary syndrome (PCOS) induced by Letrozole, the inhibition of hepatic synthesis, promotion of ovarian follicle immaturity, and elevation of androgen secretions result in an increase in the weight of the liver and ovaries. The weight of endocrine organs exhibited a decrease across all treatment groups. The histopathological examination of PCOS specimens revealed an increased presence of cysts and theca lutein cells. The group of rats with polycystic ovary syndrome (PCOS) that did not receive treatment exhibited a higher number of cysts compared to the groups that received treatment.
CONCLUSIONS: This study demonstrated that the administration of Letrozole orally resulted in the development of polycystic ovarian disease. The results indicated heightened levels of blood glucose, total cholesterol, and triglycerides, as well as alterations in hormone levels such as increased testosterone and estrogen, and decreased progesterone. These hormonal changes were accompanied by menstrual irregularities, which were confirmed through the examination of vaginal smears and histopathological analysis of the ovaries in the control group with polycystic ovarian disease. The treatment groups that received Agaricus subrufescens exhibited a decrease in blood glucose, total cholesterol, and testosterone levels.