组蛋白乙酰化是一种重要的表观遗传机制,已被证明在滞育调节中起作用。探讨组蛋白去乙酰化酶在滞育过程中的生理和分子机制,LC-MS/MS分析用于对非滞育(ND)进行TMT蛋白质组学和代谢组学分析,滞育前(PreD),滞育(D),冷疗(CT),和草甸蛾的滞育后(RD)阶段。通过蛋白质组学共鉴定出5367种蛋白质,包括1179种差异表达蛋白。我们发现975(602上调,373下调),997(608上调和389下调),1119(726个上调和393个下调),1179(上调630,下调549),94(51个上调,43个下调),111(63个上调,48个下调),533(243上调和290下调),58(31个上调,27个下调),ND和PreD中的516种(228种上调和288种下调)蛋白,ND和D,ND和CT,ND和RD,PreD和D,PreD和CT,PreD和RD,D和CT,D和RD,以及CT和RD阶段,分别。通过代谢组学注释总共1255种差异表达的代谢物。通过KEGG分析和差异表达代谢物的时间序列分析,我们发现磷脂被注释在显著不同的模块中,证明了它们在草甸蛾滞育过程中的重要作用。使用磷脂作为加权基因共表达网络分析的指标,我们分析了模块中最相关的差异表达蛋白,发现核糖体40s和60s亚基是与滞育最相关的蛋白。因为有研究表明组蛋白脱乙酰酶与草甸蛾的滞育有关,我们相信组蛋白去乙酰化酶调节核糖体的40和60亚基,进而影响草甸蛾的滞育。这一发现扩展了我们对草甸蛾滞育调控的理解,并为其调控机制提供了新的见解。
Histone acetylation is an important epigenetic mechanism that has been shown to play a role in diapause regulation. To explore the physiological and molecular mechanisms of histone deacetylase in the diapause process, LC-MS/MS analysis was used to perform TMT proteomic and metabolomic analysis on non-diapause (ND), pre-diapause (PreD), diapause (D), cold treatment (CT), and post-diapause (RD) stages of the meadow moth. A total of 5367 proteins were identified by proteomics, including 1179 differentially expressed proteins. We found 975 (602 up-regulated and 373 down-regulated), 997 (608 up-regulated and 389 down-regulated), 1119 (726 up-regulated and 393 down-regulated), 1179 (630 up-regulated and 549 down-regulated), 94 (51 up-regulated and 43 down-regulated), 111 (63 up-regulated and 48 down-regulated), 533 (243 up-regulated and 290 down-regulated), 58 (31 up-regulated and 27 down-regulated), and 516 (228 up-regulated and 288 down-regulated) proteins in ND and PreD, ND and D, ND and CT, ND and RD, PreD and D, PreD and CT, PreD and RD, D and CT, D and RD, and CT and RD stages, respectively. A total of 1255 differentially expressed metabolites were annotated by metabolomics. Through KEGG analysis and time series analysis of differentially expressed metabolites, we found that phospholipids were annotated in significantly different modules, demonstrating their important role in the diapause process of the meadow moth. Using phospholipids as an indicator for weighted gene co-expression network analysis, we analyzed the most relevant differentially expressed proteins in the module and found that ribosomal 40s and 60s subunits were the most relevant proteins for diapause. Because there have been studies that have shown that histone deacetylase is associated with the diapause of meadow moths, we believe that histone deacetylase regulates the 40s and 60s subunits of ribosomes, which in turn affects the diapause of meadow moths. This finding expands our understanding of the regulation of meadow moth diapause and provides new insights into its control mechanism.