目的:1型糖尿病与线粒体功能障碍有关。然而,成人这种功能障碍的机制尚不清楚.
方法:使用来自多项临床试验的数据进行二次分析,这些试验测量了1型糖尿病成人(n=34,年龄38.8±14.6岁)和类似年龄的对照组(n=59,年龄44.6±13.9岁)体内和体外线粒体功能。之前评估了体内线粒体功能,during,在用31磷磁共振波谱进行等距运动后。使用股外侧肌组织的高分辨率呼吸测定法来评估离体测量。
结果:体内数据显示出较高的无氧糖酵解率(p=0.013),1型糖尿病成年人的最大线粒体氧化能力(p=0.012)和线粒体效率(p=0.024)较低。调整年龄和体脂百分比后,1型糖尿病成年人的最大线粒体容量(p=0.014)继续较低,无氧糖酵解较高(p=0.040)。离体数据没有显示两组之间的显著差异。
结论:体内分析表明,患有1型糖尿病的成年人具有线粒体功能障碍。这建立在先前的研究显示1型糖尿病青少年体内线粒体功能障碍的基础上,并表明底物或氧气输送缺陷可能在体内功能障碍中起作用。
OBJECTIVE: Type 1 diabetes has been associated with mitochondrial dysfunction. However, the mechanism of this dysfunction in adults remains unclear.
METHODS: A secondary analysis was conducted using data from several clinical trials measuring in-vivo and ex-vivo mitochondrial function in adults with type 1 diabetes (n = 34, age 38.8 ± 14.6 years) and similarly aged controls (n = 59, age 44.6 ± 13.9 years). In-vivo mitochondrial function was assessed before, during, and after isometric exercise with 31phosphorous magnetic resonance spectroscopy. High resolution respirometry of vastus lateralis muscle tissue was used to assess ex-vivo measures.
RESULTS: In-vivo data showed higher rates of anaerobic glycolysis (p = 0.013), and a lower maximal mitochondrial oxidative capacity (p = 0.012) and mitochondrial efficiency (p = 0.024) in adults with type 1 diabetes. After adjustment for age and percent body fat maximal mitochondrial capacity (p = 0.014) continued to be lower and anaerobic glycolysis higher (p = 0.040) in adults with type 1 diabetes. Ex-vivo data did not demonstrate significant differences between the two groups.
CONCLUSIONS: The in-vivo analysis demonstrates that adults with type 1 diabetes have mitochondrial dysfunction. This builds on previous research showing in-vivo mitochondrial dysfunction in youths with type 1 diabetes and suggests that defects in substrate or oxygen delivery may play a role in in-vivo dysfunction.