本研究旨在检查螺旋藻补充对pro/抗氧化剂状态的影响,精英橄榄球运动员力竭运动后立即和24小时的炎症和骨骼肌损伤标志物。
在双盲设计中,17名优秀的男性橄榄球联盟运动员被随机分配到螺旋藻组(SPI:n=9)或安慰剂组(PLA:n=8)。受试者补充螺旋藻(5.7g第1天)或安慰剂(等蛋白质和热量)7周。在基线和补充7周后,在(T0)之前获得血液样本,紧后(T1)和24小时后(T2)力竭运动。Yoyo间歇性恢复测试2级用作引起氧化应激(OS)的详尽练习,炎症和骨骼肌损伤。研究的参数包括促/抗氧化剂状态标志物(超氧化物歧化酶,谷胱甘肽过氧化物酶,还原谷胱甘肽/谷胱甘肽二硫化物比,氧化低密度脂蛋白和F2α-异前列腺素[F2-Isop]),炎症标志物(髓过氧化物酶和C反应蛋白[CRP])和骨骼肌损伤标志物(乳酸脱氢酶和肌酸激酶[CK])。
我们的结果显示F2-Isop,仅PLA组的CRP和CK水平在T1时显着增加(分别为p<0.05,p<0.05和p<0.001),SPI组无变化,这反映了螺旋藻预防脂质过氧化的作用,力竭运动引起的炎症和骨骼肌损伤。此外,考虑到F2-Isop,补充螺旋藻加速了向基线值的恢复,SPI组T2时CRP和CK水平显著低于T0时(分别为p<0.05、p<0.01和p<0.001)。
根据本研究中使用的标记,我们的结果表明,补充螺旋藻可能防止运动引起的脂质过氧化,炎症和骨骼肌损伤,也可能加速其中一些标志物的恢复。根据我们的发现,我们建议补充螺旋藻,特别是对于那些没有达到推荐的抗氧化剂饮食摄入量并且执行旨在降低OS大小的高训练负荷的运动员,炎症和骨骼肌损伤,这可以帮助减少性能损失并在整个赛季的训练/比赛后加速恢复。
The present study aimed to examine the effects of spirulina supplementation on pro/antioxidant status, inflammation and skeletal muscle damage markers immediately and 24 h after exhaustive exercise in elite rugby players.
Seventeen elite male Rugby Union players were randomly assigned to a spirulina (SPI: n = 9) or placebo (PLA: n = 8) group in a double-blind design. Subjects were supplemented with Spirulina platensis (5.7 g day-1 ) or placebo (isoproteic and caloric) for 7 weeks. At baseline and after 7 weeks of supplementation, blood samples were obtained before (T0), immediately after (T1) and 24 h after (T2) exhaustive exercise. The Yoyo Intermittent Recovery Test Level 2 was used as an exhaustive exercise to induce oxidative stress (OS), inflammation and skeletal muscle damage. The studied parameters included pro/antioxidant status markers (superoxide dismutase, glutathione peroxidase, reduced glutathione/glutathione disulphide ratio, oxidised low-density lipoprotein and F2α-isoprostanes [F2-Isop]), inflammation markers (myeloperoxidase and C-reactive protein [CRP]) and skeletal muscle damage markers (lactate dehydrogenase and creatine kinase [CK]).
Our results showed that F2-Isop, CRP and CK levels significantly increased at T1 only in the PLA group (p < 0.05, p < 0.05 and p < 0.001, respectively) with no change in the SPI group, which reflects the effect of spirulina to prevent lipid peroxidation, inflammation and skeletal muscle damage induced by exhaustive exercise. Moreover, spirulina supplementation accelerated the return to baseline values given that F2-Isop, CRP and CK levels at T2 were significantly lower than at T0 in the SPI group (p < 0.05, p < 0.01 and p < 0.001, respectively).
Based on the markers used in the present study, our results show that spirulina supplementation potentially prevents exercise-induced lipid peroxidation, inflammation and skeletal muscle damage, and may also accelerate the recovery of some of these markers. Based on our findings, we recommend spirulina supplementation especially for those athletes who do not achieve the recommended antioxidant dietary intake and who perform a high training load aiming to reduce the magnitude of OS, inflammation and skeletal muscle damage, which could help to reduce performance losses and accelerate recovery after training/competitions throughout the season.