目的:确定在美国火烈鸟(Phoenicopterusruber)中单次SC剂量后高浓度丁丙诺啡制剂的药代动力学参数。
方法:6只健康的成年美洲火烈鸟(3只雄性和3只雌性)。
方法:向所有禽类SC施用单剂量的高浓度丁丙诺啡(1.8mg/kg)。在2022年10月14日至10月18日之间的药物施用后0.25、0.5、1、2、4、8、12、24、48、72和96小时收集血液样品。通过液相色谱-串联质谱法测定血浆丁丙诺啡浓度,并使用非房室分析确定药代动力学参数。
结果:平均±SD峰值血浆药物浓度(Cmax)为195.1±187.4ng/mL,平均血浆浓度峰值时间(Tmax)为0.32±0.31小时,从时间0到最后一次测量浓度(AUC0-last)的浓度-时间曲线下的平均面积为881.4±205.4ng/mL,平均终末半衰期(t1/2)为12.6±3.86小时。给药后至少48小时,平均血浆丁丙诺啡浓度>1ng/mL。没有观察到临床上显著的不良反应。
结论:在美国火烈鸟中以1.8mg/kgSC给药的高浓度丁丙诺啡迅速超过了据报道在其他禽类中具有镇痛作用的血浆药物浓度,并在延长的时间内维持了这些水平。镇静作用与其他物种的报道相似。需要进一步的研究来评估该剂量的高浓度丁丙诺啡在美国火烈鸟中的临床疗效。
OBJECTIVE: To determine the pharmacokinetic parameters of a high-concentration buprenorphine formulation after a single SC dose in American flamingos (Phoenicopterus ruber).
METHODS: 6 healthy adult American flamingos (3 males and 3 females).
METHODS: A single dose of high-concentration buprenorphine (1.8 mg/kg) was administered SC to all birds. Blood samples were collected at 0.25, 0.5, 1, 2, 4, 8, 12, 24, 48, 72, and 96 hours after drug administration between October 14 and October 18, 2022. Plasma buprenorphine concentrations were determined by liquid chromatography-tandem mass spectrometry and a noncompartmental analysis was used to determine pharmacokinetic parameters.
RESULTS: Mean ± SD peak plasma drug concentration (Cmax) was 195.1 ± 187.4 ng/mL, the mean time to peak plasma concentration (Tmax) was 0.32 ± 0.31 hours, the mean area under the concentration-vs-time curve from time 0 to the last measured concentration (AUC0-last) was 881.4 ± 205.4 ng/mL, and mean terminal half-life (t1/2) was 12.6 ± 3.86 hours. Mean plasma buprenorphine concentrations were >1 ng/mL for at least 48 hours after drug administration. No clinically significant adverse effects were observed.
CONCLUSIONS: High-concentration buprenorphine dosed at 1.8 mg/kg SC in American flamingos rapidly exceeded plasma drug concentrations reported to have analgesic effects in other avian species and maintained these levels for extended periods. Sedative effects were similar to those reported for other species. Additional studies are needed to evaluate the clinical efficacy of high-concentration buprenorphine at this dose in American flamingos.