■白细胞介素13受体α2亚基(IL13RA2)在许多癌症的进展中起着至关重要的作用。然而,IL13RA2在婴幼儿血管瘤(IH)中的作用尚不清楚.
■使用蛋白质印迹分析IH组织中的IL13RA2表达,qRT-PCR,和免疫荧光。IL13RA2在血管瘤衍生的内皮细胞(HemECs)中的作用是在使用CCK-8,集落形成,凋亡,伤口愈合,小管形成,Transwell,和westernblot.
■IL13RA2在IH组织中表达上调。IL13RA2过表达促进增殖,迁移,以及HemEC的入侵和诱导的糖酵解,用糖酵解抑制剂证实了这一点。具体来说,IL13RA2与β-catenin相互作用并激活HemECs中的Wnt/β-catenin通路,参与IL13RA2的上述作用。
■这些发现揭示了靶向IL13RA2是治疗IH的潜在方法。
UNASSIGNED: Interleukin 13 receptor subunit alpha 2 (IL13RA2) plays an essential role in the progression of many cancers. However, the role of IL13RA2 in infantile haemangioma (IH) is still unknown.
UNASSIGNED: IL13RA2 expression in IH tissues was analyzed using western blot, qRT-PCR, and immunofluorescence. The role of IL13RA2 in haemangioma-derived endothelial cells (HemECs) was determined following knockdown or overexpression of IL13RA2 using CCK-8, colony formation, apoptosis, wound healing, tubule formation, Transwell, and western blot.
UNASSIGNED: IL13RA2 expression was upregulated in IH tissues. IL13RA2 overexpression promoted proliferation, migration, and invasion of HemECs and induced glycolysis, which was confirmed with a glycolysis inhibitor. Specifically, IL13RA2 interacted with β-catenin and activated the Wnt/β-catenin pathway in HemECs, which were involved in the above-mentioned effects of IL13RA2.
UNASSIGNED: These findings revealed that targeting IL13RA2 is a potential therapeutic approach for IH.