土壤盐渍化正在对全球土壤产生负面影响,由于土壤资源的功能和利益丧失,这一问题的蔓延备受关注。在本研究中,我们探索了Villamar的地热地区\“LosNegritos\”附近的没有农业实践的钠盐土壤和两种具有农业实践的土壤(一种钠和一种盐)的耕地中嗜盐和耐盐微生物的多样性,米却肯州。这是通过他们的分离和分子鉴定来实现的,以及在盐水条件下生产具有生物技术意义的代谢物和酶的潜力的表征。使用依赖于文化的技术,属于芽孢杆菌属的62株耐盐和中度嗜盐菌株,短杆菌属,Gracilibacillus,Halobacillus,Halomonas,Kocuria,Marinococus,Nesterenkonia,海洋芽孢杆菌,Planococcus,Priestia,唾液,抗菌,Salinicocus,葡萄球菌,Terribacillus,并分离了病毒杆菌。不同菌株在15%(w/v)的盐下合成水解酶,以及具有促进植物生长(PGP)特征的代谢物,如吲哚乙酸(IAA),在盐水条件下。此外,在菌株中检测到生物聚合物的产生;芽孢杆菌的成员,Halomonas,葡萄球菌,和盐霉素显示胞外聚合物(EPS)的产生,和菌株Halomonassp.LNSP3E3-1.2在10%(w/v)的总盐下产生聚羟基丁酸酯(PHB)。
Soil salinization is negatively affecting soils globally, and the spread of this problem is of great concern due to the loss of functions and benefits offered by the soil resource. In the present study, we explored the diversity of halophilic and halotolerant microorganisms in the arable fraction of a sodic-saline soil without agricultural practices and two soils with agricultural practices (one sodic and one saline) near the geothermal area \"Los Negritos\" in Villamar, Michoacán state. This was achieved through their isolation and molecular identification, as well as the characterization of their potential for the production of metabolites and enzymes of biotechnological interest under saline conditions. Using culture-dependent techniques, 62 halotolerant and moderately halophilic strains belonging to the genera Bacillus, Brachybacterium, Gracilibacillus, Halobacillus, Halomonas, Kocuria, Marinococcus, Nesterenkonia, Oceanobacillus, Planococcus, Priestia, Salibactetium, Salimicrobium, Salinicoccus, Staphylococcus, Terribacillus, and Virgibacillus were isolated. The different strains synthesized hydrolytic enzymes under 15% (w/v) of salts, as well as metabolites with plant-growth-promoting (PGP) characteristics, such as indole acetic acid (IAA), under saline conditions. Furthermore, the production of biopolymers was detected among the strains; members of Bacillus, Halomonas, Staphylococcus, and Salinicoccus showed extracellular polymeric substance (EPS) production, and the strain Halomonas sp. LNSP3E3-1.2 produced polyhydroxybutyrate (PHB) under 10% (w/v) of total salts.