本研究的重点是从芳香草的根际区即palmarosa(Cymbopogonmartinii(Roxb。Wats),柠檬草(Cymbopogonflexuosus)和香根草(Chrysopogonzizaniodes(L.)纳什。).因此,这些分离物单独或与芳香草的植被结合将用于清理受CP污染的土壤。该研究还探索了酶活性,CO2释放,脱氯潜力,和细菌菌株的降解途径。共分离出53株CP耐性细菌,其物理特性和降解CP的能力。10株对CP高度耐受的分离株分别为铜绿假单胞菌Pa608,3株来自不同根际的木偶假单胞菌R4-721,凝集素肠球菌PP2a,假单胞菌NBFPALD_RAS131,阴沟肠杆菌L3,嗜麦芽窄食单胞菌PEG-390,大肠杆菌ABRL132和大肠杆菌O104:H4菌株FWSEC0009。CP培养基中分离物的CO2排放和磷酸酶活性分别在3.1至8.6μmolmL-1和12.3至31μmolPNPh-1之间变化。这些分离物的CP降解动力学遵循单相衰减模型,耗散率范围为0.048至0.41d-1,半衰期为1.7-14.3天。在SGompertz方程中拟合的生长数据显示,生长速率(K)为0.21±0.28至0.91±0.33d-1。在分离株中,Monteilii菌株具有较快的生长速率,而大肠杆菌ABRL132具有较慢的生长。通过SGompertz方程计算的TCP积累速率为0.21±0.02至1.18±0.19d-1。单单胞菌显示出较低的TCP积累速率。其中,4个高效分离株分别为铜绿假单胞菌Pa608、假单胞菌NBFPALD_RAS131、嗜麦芽窄食单胞菌PEG-390和假单胞菌hibiscicolaR4-721。降解途径的说明表明,每个分离物的代谢途径的差异与它们的生长速率有关,磷酸酶脱氢酶,氧化酶,和脱氯活动。
The present study focused on the isolation and identification of CP and TCP bacteria degrading bacteria from the rhizospheric zone of aromatic grasses i.e. palmarosa (Cymbopogon martinii (Roxb. Wats), lemongrass (Cymbopogon flexuosus) and vetiver (Chrysopogon zizaniodes (L.) Nash.). So that these isolates alone or in combination with the vegetation of aromatic grasses will be used to clean up CP-contaminated soils. The study also explored enzymatic activities, CO2 release, dechlorination potential, and degradation pathways of bacterial strains. A total of 53 CP-tolerant bacteria were isolated on their physical characteristics and their ability to degrade CP. The ten highly CP-tolerant isolates were Pseudomonas aeruginosa Pa608, three strains of Pseudomonas hibiscicola R4-721 from different rhizosphere, Enterococcus lectis PP2a, Pseudomonas monteilii NBFPALD_RAS131, Enterobacter cloacae L3, Stenotrophomonas maltophilia PEG-390, Escherichia coli ABRL132, and Escherichia coli O104:H4 strain FWSEC0009. The CO2 emission and phosphatase activities of the isolates varied from 3.1 to 8.6 μmol mL-1 and 12.3 to 31 μmol PNP h-1, respectively in the CP medium. The degradation kinetics of CP by these isolates followed a one-phase decay model with a dissipation rate ranging from 0.048 to 0.41 d-1 and a half-life of 1.7-14.3 days. The growth data fitted in the SGompertz equation showed a growth rate (K) of 0.21 ± 0.28 to 0.91 ± 0.33 d-1. The P. monteilii strain had a faster growth rate while E. coli ABRL132 had slower growth among the isolates. The rate of TCP accumulation calculated by the SGompertz equation was 0.21 ± 0.02 to 1.18 ± 0.19 d-1. The Pseudomonas monteilii showed a lower accumulation rate of TCP. Among these, four highly effective isolates were Pseudomonas aeruginosa Pa608, Pseudomonas monteilii NBFPALD_RAS131, Stenotrophomonas maltophilia PEG-390, and Pseudomonas hibiscicola R4-721. Illustrations of the degradation pathways indicated that the difference in metabolic pathways of each isolate was associated with their growth rate, phosphatase, dehydrogenase, oxidase, and dechlorination activities.