背景:肠道病毒与炎症性肠病(IBD)有关,然而,充分了解IBD患者的肠道病毒,尤其是在不同的地理人群中,缺乏。
结果:在这项研究中,我们在一个由71例IBD患者(15例克罗恩病患者和56例溃疡性结肠炎患者)和77例健康对照者组成的中国队列中,通过对其粪便进行病毒样颗粒(VLP)和批量病毒测序,进行了一项全面的肠道病毒学范围关联研究.通过利用针对IBD病毒的集成肠道病毒目录,我们揭示了IBD患者肠道病毒的根本改变。这些表征了139个差异丰富的病毒特征,包括预测感染埃希氏菌的噬菌体升高,克雷伯菌属,肠球菌B,链球菌,和Veillonella物种,以及针对普雷沃氏菌的IBD耗尽噬菌体,Ruminococcus_E,双歧杆菌,和Blautia物种。值得注意的是,这些病毒特征在欧洲和美国等不同人群中表现出高度的一致性,强调它们在疾病背景下的重要性和广泛的相关性。此外,粪便病毒移植实验证实,这些IBD表征的病毒的定植可以调节小鼠模型中的实验性结肠炎。
结论:基于对IBD肠道病毒的这些见解,我们确定了IBD患者预后和治疗的潜在生物标志物,为进一步探索相关条件下的病毒学奠定基础。视频摘要。
BACKGROUND: The gut virome has been implicated in inflammatory bowel disease (IBD), yet a full understanding of the gut virome in IBD patients, especially across diverse geographic populations, is lacking.
RESULTS: In this study, we conducted a comprehensive gut virome-wide association study in a Chinese cohort of 71 IBD patients (15 with Crohn\'s disease and 56 with ulcerative colitis) and 77 healthy controls via viral-like particle (VLP) and bulk virome sequencing of their feces. By utilizing an integrated gut virus catalog tailored to the IBD virome, we revealed fundamental alterations in the gut virome in IBD patients. These characterized 139 differentially abundant viral signatures, including elevated phages predicted to infect Escherichia, Klebsiella, Enterococcus_B, Streptococcus, and Veillonella species, as well as IBD-depleted phages targeting Prevotella, Ruminococcus_E, Bifidobacterium, and Blautia species. Remarkably, these viral signatures demonstrated high consistency across diverse populations such as those in Europe and the USA, emphasizing their significance and broad relevance in the disease context. Furthermore, fecal virome transplantation experiments verified that the colonization of these IBD-characterized viruses can modulate experimental colitis in mouse models.
CONCLUSIONS: Building upon these insights into the IBD gut virome, we identified potential biomarkers for prognosis and therapy in IBD patients, laying the foundation for further exploration of viromes in related conditions. Video Abstract.