小松鼠(Spermophiluspygmaeus)的年度周期包括2个时期:觉醒(春秋)和冬眠(冬季)。在活动期间,地松鼠品种(春季),积累脂肪储备(夏季),准备冬眠(秋季)。我们假设血液的流变学特性和红细胞的变形性可以在动物觉醒期的不同季节发生变化,从而为组织充分提供氧气。这项研究的目的是确定地松鼠活动期间红细胞变形能力和红细胞指数的可能适应性变化。在渗透梯度中通过ektacytometry法研究了红细胞的变形性。人们发现,在春天,地松鼠被唤醒后,红细胞具有最高的可变形性(El_max),水合(O_hyper),透水性(El_min)和渗透稳定性(ΔO)。在夏天,与春天相比,红细胞的变形能力下降,并且红细胞的平均体积(MCV)也减少。在秋天,在冬眠之前,红细胞的整体变形能力,他们的水合作用,以及红细胞的渗透稳定性范围相对于夏季增加。在夏天和秋天,与春天相比,红细胞中血红蛋白的平均浓度(MCHC)增加。在夏季和秋季的低剪应力(1Pa)下,osmoscan成为一种明显的多模态形式,表明地松鼠红细胞膜粘弹性的变化。因此,第一次,我们发现了地松鼠红细胞变形能力的季节性变化,这与动物的春夏活动和冬眠准备是一致的。
The annual cycle of small ground squirrels (Spermophilus pygmaeus) consists of 2 periods: wakefulness (spring-autumn) and hibernation (winter). During the active period, ground squirrels breed (spring), accumulate fat reserves (summer) and prepare for hibernation (autumn). We suppose that the rheological properties of blood and the deformability of erythrocytes can change in different seasons of the animal\'s wakefulness period to adequately provide tissues with oxygen. The aim of this study was to identify possible adaptive changes in erythrocyte deformability and erythrocyte indices in ground squirrels during their active period. The deformability of erythrocytes was studied by ektacytometry in an osmotic gradient. It was found that in spring, after the arousal of ground squirrels, erythrocytes have the highest deformability (El_max), hydration (O_hyper), water permeability (El_min) and osmotic stability (∆O). In summer, compared with spring, the deformability of erythrocytes decreases, and the average volume of erythrocytes (MCV) also decreases. In autumn, before hibernation, the integral deformability of erythrocytes, their hydration, as well as the range of osmotic stability of erythrocytes increases relative to the summer period. In summer and autumn, in contrast to spring, the average concentration of hemoglobin in the erythrocyte (MCHC) increases. At low shear stress (1 Pa) in summer and autumn, osmoscan becomes a pronounced polymodal form, indicating a change in the viscoelastic properties of ground squirrel erythrocyte membranes. Thus, for the first time, we discovered seasonal variability in the deformability of ground squirrel erythrocytes, which is consistent with the spring-summer activity of animals and preparation for hibernation.