未经证实:由于缺乏可以选择性地将治疗性基因传递给癌细胞的有效纳米载体,因此阻碍了基因治疗治疗前列腺癌的使用。为了克服这一点,我们假设缀合乳铁蛋白,肿瘤靶向配体,和二氨基丁酸聚丙烯亚胺树枝状聚合物进入金纳米笼,然后与质粒DNA复合,在不使用外部刺激的情况下,将增强前列腺癌细胞中的基因表达和抗增殖活性。
UNASSIGNED:合成并表征了具有乳铁蛋白并与二氨基丁酸聚丙烯亚胺树枝状聚合物(AuNC-DAB-Lf)缀合的新型金纳米笼。与质粒DNA复合后,他们的基因表达,在PC-3前列腺癌细胞上评估细胞摄取和抗增殖功效.
未经鉴定:AuNC-DAB-Lf能够以5:1以上的缀合物:DNA重量比复合DNA。基因表达在用AuNC-DAB-Lf以10:1的重量比处理后达到最高,这是由于在PC-3细胞中以该比例由缀合物介导的DNA摄取显著增加。因此,与编码TNFα的DAB树枝状复合物相比,编码TNFα的AuNC-DAB-Lf-DNA的抗增殖活性显着提高了9倍。
UNASSIGNED:带有乳铁蛋白的树枝状聚合物缀合的金纳米笼是用于治疗前列腺癌的非常有前途的基因递送系统。
UNASSIGNED: The use of gene therapy to treat prostate cancer is hampered by the lack of effective nanocarriers that can selectively deliver therapeutic genes to cancer cells. To overcome this, we hypothesize that conjugating lactoferrin, a tumor-targeting ligand, and the diaminobutyric polypropylenimine dendrimer into gold nanocages, followed by complexation with a plasmid DNA, would enhance gene expression and anti-proliferation activity in prostate cancer cells without the use of external stimuli.
UNASSIGNED: Novel gold nanocages bearing lactoferrin and conjugated to diaminobutyric polypropylenimine dendrimer (AuNCs-DAB-Lf) were synthesized and characterized. Following complexation with a plasmid DNA, their gene expression, cellular uptake and anti-proliferative efficacies were evaluated on PC-3 prostate cancer cells.
UNASSIGNED: AuNCs-DAB-Lf was able to complex DNA at conjugate: DNA weight ratios 5:1 onwards. Gene expression was at its highest after treatment with AuNCs-DAB-Lf at a weight ratio of 10:1, as a result of a significant increase in DNA uptake mediated by the conjugate at that ratio in PC-3 cells. Consequently, the anti-proliferative activity of AuNCs-DAB-Lf-DNA encoding TNFα was significantly improved by up to 9-fold compared with DAB dendriplex encoding TNFα.
UNASSIGNED: Lactoferrin-bearing dendrimer-conjugated gold nanocages are highly promising gene delivery systems for the treatment of prostate cancer.