在这项研究中,研究了枸杞提取物(LSB)在大鼠中的抗糖尿病活性。使用α-淀粉酶评估LSB的体外抗糖尿病特性,α-葡萄糖苷酶和DPP-IV抑制试验,而抗氧化剂测定是使用DPPH分析的,ABTS和FRAP测定。2型糖尿病是高果糖/链脲佐菌素,糖尿病动物用LSB治疗5周。实验结束时,通过胰岛素水平评估LSB的效果,血脂谱和肝肾功能生物标志物。氧化炎症参数的水平,评估胰腺的组织病理学和胰岛素免疫组织化学染色。糖尿病大鼠血糖水平显著升高,食物/水的摄入量,脂质分布,肝肾功能生物标志物,以及体重和血清胰岛素水平显着下降。组织病理学和胰岛素免疫组织化学检查也显示胰腺β细胞和胰岛素阳性细胞减少,分别。这些改变与丙二醛的显著增加有关,TNF-α和IL-1β,除了GSH的大幅下降之外,SOD和CAT活性。LSB显著降低血糖水平,葡萄糖不耐受,血脂,在治疗的糖尿病大鼠中恢复了改变的肝肾和胰腺功能。Further,LSB通过减少丙二醛表现出抗氧化和抗炎活性,TNF-α,IL-1β,并增加胰腺组织中的抗氧化酶活性。在LSB的UPLC-Q-TOF-MS分析中初步鉴定了总共77种次生代谢物。总的来说,这些发现提供了深入了解LSB作为抗糖尿病药的潜力,这可能与植物中过多的生物活性化合物有关。
In this study, the antidiabetic activities of Lepionurus sylvestris Blume extract (LSB) in rats was investigated. The in vitro antidiabetic properties of LSB was evaluated using α-amylase, α-glucosidase and DPP-IV inhibitory assays, while the antioxidant assay was analysed using DPPH, ABTS and FRAP assays. Type 2 diabetes was with high-fructose/streptozotocin, and the diabetic animals were treated with LSB for 5 weeks. At the end of the experiment, the effects of LSB were evaluated via insulin level, lipid profile and hepatorenal function biomarkers. The level of oxido-inflammatory parameters, histopathology and insulin immunohistochemical staining in the pancreas was evaluated. Diabetic rats manifested significant increases in the blood glucose level, food/water intake, lipid profiles, hepatorenal function biomarkers, as well as a marked decreases in the body weight and serum insulin levels. Histopathological and insulin immunohistochemical examination also revealed decreased pancreatic beta cells and insulin positive cells, respectively. These alterations were associated with significant increases in malondialdehyde, TNF-α and IL-1β, in addition to significant declines in GSH, SOD and CAT activities. LSB significantly reduced blood glucose level, glucose intolerance, serum lipids, restored altered hepatorenal and pancreatic functions in the treated diabetic rats. Further, LSB showed antioxidant and anti-inflammatory activities by reducing malondialdehyde, TNF-α, IL-1β, and increasing antioxidant enzymes activities in the pancreatic tissues. A total of 77 secondary metabolites were tentatively identified in the UPLC-Q-TOF-MS analysis of LSB. Overall, these findings provides insight into the potentials of LSB as an antidiabetic agent which may be associated to the plethora bioactive compounds in the plant.