背景:未知功能域247(DUF247)蛋白参与植物发育和胁迫反应。水稻是重要的世界性谷类作物,尽管已经鉴定出越来越多的DUF蛋白,水稻对DUF蛋白的认识还很有限。
结果:在这项研究中,通过同源性搜索和结构域预测,我们在水稻(Oryzasativa)基因组中鉴定了69个编码DUF247蛋白的基因。所有OsDUF247蛋白被分为四个主要组(I,II,III和IV)通过系统发育分析。值得注意的是,聚集在染色体上的OsDUF247基因仅显示出紧密的系统发育关系,这表明基因重复驱动了水稻基因组中DUF247基因家族的扩展。组织谱分析表明,大多数DUF247基因在幼苗中以组成水平表达,根,茎,和叶子,除了七个基因(LOC_Os01g21670,LOC_Os03g19700,LOC_Os05g04060,LOC_Os08g26820,LOC_Os08g26840,LOC_Os08g26850和LOC_Os09g13410)外。这七个基因是由各种非生物胁迫诱导的,包括寒冷,干旱,热,激素治疗,尤其是盐,如进一步的实验分析表明。DUF247蛋白含有位于膜上的跨膜结构域,表明它们在水稻发育和适应环境中的重要作用。
结论:这些发现为DUF247基因的功能表征奠定了基础,以揭示其在水稻品种中的确切作用。
BACKGROUND: The domain of unknown function 247 (DUF247) proteins is involved in plant development and stress response. Rice is an important worldwide cereal crop, although an increasing number of DUF proteins have been identified, the understanding of DUF proteins is still very limited in rice.
RESULTS: In this study, we identified 69 genes that encode DUF247 proteins in the rice (Oryza sativa) genome by homology searches and domain prediction. All the OsDUF247 proteins were classified into four major groups (I, II, III and IV) by phylogenetic analysis. Remarkably, OsDUF247 genes clustered on the chromosomes solely show close phylogenetic relationships, suggesting that gene duplications have driven the expansion of the DUF247 gene family in the rice genome. Tissue profile analysis showed that most DUF247 genes expressed at constitutive levels in seedlings, roots, stems, and leaves, except for seven genes (LOC_Os01g21670, LOC_Os03g19700, LOC_Os05g04060, LOC_Os08g26820, LOC_Os08g26840, LOC_Os08g26850 and LOC_Os09g13410) in panicles. These seven genes were induced by various abiotic stress, including cold, drought, heat, hormone treatment, and especially salt, as demonstrated by further experimental analysis. DUF247 proteins contain transmembrane domains located on the membrane, suggesting their significant roles in rice development and adaptation to the environment.
CONCLUSIONS: These findings lay the foundation for functional characterizations of DUF247 genes to unravel their exact role in rice cultivars.