弯曲杆菌病,食源性疾病,是世界上胃肠道疾病的主要原因之一。这项研究调查了人类弯曲杆菌病与空肠弯曲杆菌潜在污染食品的消费之间的联系。从人类身上收集了三百六十份样本,鸡肉泄殖腔,生鸡肉,未经巴氏灭菌的牛奶,和蔬菜。这些鸡只获自持牌及非持牌屠房,只研究了脖子和翅膀。在微氧条件下富集样品,然后在改良的木炭头孢哌酮脱氧胆酸盐琼脂上培养。通过染色鉴定细菌,生化检测,并通过聚合酶链反应对毒力基因进行分子鉴定;hipO,asp,dnaJ,cadF,cdtA,cdtB,和cdtC。通过血清学Penner测试和脉冲场凝胶电泳(PFGE)评估了人和鸡分离株之间空肠弯曲杆菌的基因组同质性。在蔬菜和巴氏杀菌牛奶中未检测到弯曲杆菌,虽然,根据形态推测,仅有20株来自鸡和临床样本的分离株为弯曲杆菌.生化试验证实5个分离株为大肠杆菌,15个分离株是空肠杆菌,包括两个来自人类的分离株,剩下的是鸡。与从非许可屠宰场获得的(33.3%)相比,从许可屠宰场获得的鸡的脖子上的空肠弯曲杆菌定殖明显较低(6.66%)。药敏试验表明,所有鉴定出的空肠弯曲杆菌对抗生素均具有耐药性,大多数分离株(53.5%)对六种抗生素表现出耐药性,虽然,所有分离株均对环丙沙星耐药,四环素,还有氨曲南.Penner检验显示P:21为人类分离株的优势血清型,脖子,还有Cloaca.人类分离株和鸡颈中空肠弯曲杆菌的血清同源性,翅膀,泄殖腔占71%,36%,78%,分别。限制性内切酶Smal对DNA片段化模式的PFGE分析显示,与与泄殖腔分离株的部分同源性相比,空肠弯曲杆菌人分离株和鸡颈具有完全的基因型同源性。该研究提请注意需要有效的干预措施,以确保商业食物链供应的安全家禽生产的最佳做法,以限制食源性病原体的感染,包括弯曲杆菌.
Campylobacteriosis, a foodborne illness, is one of the world\'s leading causes of gastrointestinal illness. This study investigates the link between human campylobacteriosis and the consumption of potentially contaminated food with Campylobacter jejuni. Three hundred sixty samples were collected from humans, chicken cloaca, raw chicken meat, unpasteurized milk, and vegetables. The chickens were obtained from licensed and non-licensed slaughterhouses, and only the necks and wings were studied. Samples were enriched under microaerobic conditions then cultured on the modified charcoal cefoperazone deoxycholate agar. Bacteria was identified by staining, biochemical testing, and molecular identification by the polymerase chain reaction for the virulence genes; hipO, asp, dnaJ, cadF, cdtA, cdtB, and cdtC. The genomic homogeneity of C. jejuni between human and chicken isolates was assessed by the serological Penner test and the pulse field gel electrophoresis (PFGE). Campylobacter was not detected in the vegetables and pasteurized milk, though, only twenty isolates from chickens and clinical samples were presumed to be Campylobacter based on their morphology. The biochemical tests confirmed that five isolates were C. coli, and fifteen isolates were C. jejuni including two isolates from humans, and the remaining were from chickens. The colonization of C. jejuni in chickens was significantly lower in necks (6.66%) obtained from licensed slaughterhouses compared to those obtained from non-licensed slaughterhouses (33.3%). The antimicrobial susceptibility test showed that all identified C. jejuni isolates were resistant to antibiotics, and the majority of isolates (53.5%) showed resistance against six antibiotics, though, all isolates were resistant to ciprofloxacin, tetracycline, and aztreonam. The Penner test showed P:21 as the dominant serotype in isolates from humans, necks, and cloaca. The serohomology of C. jejuni from human isolates and chicken necks, wings, and cloaca was 71%, 36%, 78%, respectively. The PFGE analysis of the pattern for DNA fragmentation by the restriction enzyme Smal showed a complete genotypic homology of C. jejuni human isolates and chicken necks compared to partial homology with cloacal isolates. The study brings attention to the need for effective interventions to ensure best practices for safe poultry production for commercial food chain supply to limit infection with foodborne pathogens, including Campylobacter.