■进行后处理(PP)以改善表面,这可能有利于微生物粘附和随之而来的病理表现,从而损害了通过熔丝制造(FFF)获得的聚乳酸(PLA)用于生物医学应用的适应症。这旨在评估化学品的影响,热,和机械PP对链球菌突变体和白色念珠菌的粘附,粗糙度,以及通过FFF在有和没有热老化的情况下获得的PLA的润湿性。
■在3D建模程序中设计并打印了标本。化学PP通过浸入氯仿中进行,通过退火方法加热,和机械抛光。通过将温度从5°C交替到55°C进行5000个循环的热老化。对白色念珠菌和变形链球菌的双物种生物膜进行集落形成单位(CFU/mL)计数。粗糙度通过粗糙度仪分析,润湿性通过固滴技术分析。使用Shapiro-Wilk检验验证数据的正态,应用于CFU和润湿性的双向方差分析(α=0.05),粗糙度和Kruskal-Wallis(α=0.05)。
■化学,热,和机械PP方法对白色念珠菌(p=0.296)和变形链球菌(p=0.055)的CFU/mL没有影响。热老化不影响微生物粘附。化学PP具有较低的粗糙度,老化后增加了。机械PP的润湿性较低。
■后处理技术,对FFF获得的PLA中变形链球菌和白色念珠菌的粘附没有影响,化学PP降低粗糙度,和机械降低的润湿性。热老化不会改变微生物的粘附性,也不会改变粗糙度和润湿性。
UNASSIGNED: Post-processing (PP) is performed to improve the surface, which can favor microbial adhesion and consequent pathological manifestations that impair the indication of polylactic acid (PLA) obtained by fused filament fabrication (FFF) for biomedical applications. This aims to evaluate the influence of chemical, thermal, and mechanical PP on the adhesion of Streptococcus mutants and Candida albicans, roughness, and wettability of the PLA obtained by FFF with and without thermal aging.
UNASSIGNED: The specimens were designed in the 3D modeling program and printed. The chemical PP was performed by immersion in chloroform, the thermal by the annealing method, and the mechanical by polishing. Thermal aging was performed by alternating the temperature from 5 °C to 55 °C with 5000 cycles. Colony-forming unit (CFU/mL) counting was performed on dual-species biofilm of C. albicans and S. mutans. Roughness was analyzed by rugosimeter and wettability by the sessile drop technique. Data were verified for normality using the Shapiro-Wilk test, two-way ANOVA (α = 0.05) applied for CFU and wettability, and Kruskal-Wallis (α = 0.05) for roughness.
UNASSIGNED: Chemical, thermal, and mechanical PP methods showed no influence on CFU/mL of C. albicans (p = 0.296) and S. mutans (p = 0.055). Thermal aging did not influence microbial adhesion. Chemical PP had lower roughness, which had increased after aging. Wettability of the mechanical PP was lower.
UNASSIGNED: Post-processing techniques, do not present an influence on the adhesion of S. mutans and C. albicans in PLA obtained by FFF, chemical PP reduced roughness, and mechanical reduced wettability. Thermal aging did not alter the microbial adhesion and altered the roughness and wettability.