最近在湖南省患有严重临床疾病的猪中发现了一种新型圆环病毒,称为猪圆环病毒4型(PCV4)。中国。关于PCV4的起源和演变的报道很少,尽管一些研究人员对PCV4进行了流行病学调查,发现PCV4在猪中广泛存在。在以往研究的基础上,我们在繁殖失败的养殖狐狸和浣熊犬中检测到PCV2。为了探索PCV4基因组是否也存在于毛皮动物中,我们在2015年至2020年间发现了137例来自中国河北省毛皮动物养殖场的病例,其特征是食欲不振,嗜睡,抑郁症,流产,和不育。PCV4的总感染率为23.36%(32/137),包括20.37%(22/108)的浣熊狗,狐狸占18.75%(3/16),水貂占53.85%(7/13)。最后,我们对5株浣熊犬源PCV4菌株和1株狐狸源PCV4菌株进行了测序,其与其他代表性PCV4菌株的核苷酸同一性从96.5%到100%不等。基于PCV4菌株完整基因组的系统发育分析表明与从猪中鉴定的PCV4菌株密切相关。据我们所知,这是首次在毛皮动物中检测PCV4的研究。有趣的是,我们还在一个混合农场(同时喂养猪和浣熊犬)中鉴定了PCV4。总之,我们的发现扩展了对PCV4分子流行病学的理解,并为其跨物种传播提供了新的证据.
A novel circovirus called porcine circovirus type 4 (PCV4) was recently detected in pigs suffering from severe clinical diseases in Hunan province, China. There are few reports on the origin and evolution of PCV4, although some researchers have conducted epidemiological investigations of PCV4 and found that PCV4 is widespread in pigs. Based on the previous study, we detected PCV2 in farmed foxes and raccoon dogs with reproductive failure. To explore whether the PCV4 genome also exists in fur animals, we detected 137 cases admitted from fur animal farms in Hebei China between 2015 and 2020, which were characterized by inappetence, lethargy, depression, abortion, and sterility. The overall infection rate of PCV4 was 23.36% (32/137), including 20.37% (22/108) for raccoon dogs, 18.75% (3/16) for foxes, and 53.85% (7/13) for minks. Finally, five raccoon dog-origin PCV4 strains and one fox-origin PCV4 strain were sequenced in our study, whose nucleotide identities with other representative PCV4 strains varied from 96.5% to 100%. Phylogenetic analysis based on the complete genomes of PCV4 strains indicated a close relationship with those of PCV4 strains identified from pigs. To our knowledge, this is the first study to detect PCV4 in fur animals. Interestingly, we also identified PCV4 in a mixed farm (feeding pigs and raccoon dogs at the same time). In summary, our findings extend the understanding of the molecular epidemiology of PCV4 and provide new evidence for its cross-species transmission.