目的：描述年龄相关性黄斑变性（AMD）开始时大型假晶状体队列中的基线定量（短波长）自发荧光（qAF）发现；评估qAF8作为结果测量并评估AREDS和Beckman分级系统。
方法：在ALSTAR2基线队列（NCT04112667）中，188人（74.0±5.5岁）的346只假晶状体眼被分类为正常（AREDS为N=160，158Beckman），早期（e）AMD（N=104，66），和中间体(i)AMD(N=82,122)。通过6°-8°环（qAF8）中的平均qAF强度以及观察值与总体平均值之间的差异图比较组。除以标准差(Z分数)。
结果：qAF8在任一分层诊断组间没有显著差异（p=0.0869AREDS；p=0.0569Beckman）。值得注意的是,被AREDS认为是eAMD的45只眼被贝克曼变成了iAMD。对于AREDS分层的眼睛，Z-score图显示，正常情况下位于中心的qAF较高，在eAMD中接近平均值，iAMD的值较低。对于贝克曼分层的眼睛，地图偏离了这种模式。
结论：在大量的假晶状体眼样本中，qAF8从正常老化到iAMD总体上没有差异，但也没有捕获黄斑中最早的AMD活性。与Beckman分类相比，AREDS分类的结果与组织学自发荧光的缓慢下降更为一致。
BACKGROUND: The aims of the study were to describe baseline quantitative (short-wavelength) autofluorescence (qAF) findings in a large pseudophakic cohort at age-related macular degeneration (AMD)\'s beginnings and to assess qAF8 as an outcome measure and evaluate Age-Related Eye Disease Study (AREDS) and Beckman grading systems.
METHODS: In the ALSTAR2 baseline cohort (NCT04112667), 346 pseudophakic eyes of 188 persons (74.0 ± 5.5 years) were classified as normal (N = 160 by AREDS, 158 by Beckman), early AMD (eAMD) (N = 104, 66), and intermediate AMD (iAMD) (N = 82, 122). Groups were compared via mean qAF intensities in a 6°-8° annulus (qAF8) and maps of differences between observations and the overall mean, divided by standard deviation (Z-score).
RESULTS: qAF8 did not differ significantly among diagnostic groups by either stratification (p = 0.0869 AREDS; p = 0.0569 by Beckman). Notably, 45 eyes considered eAMD by AREDS became iAMD by Beckman. For AREDS-stratified eyes, Z-score maps showed higher centrally located qAF for normal, near the mean in eAMD, and lower values for iAMD. Maps deviated from this pattern for Beckman-stratified eyes.
CONCLUSIONS: In a large sample of pseudophakic eyes, qAF8 does not differ overall from normal aging to iAMD but also does not capture the earliest AMD activity in the macula lutea. AREDS classification gives results more consistent with a slow decline in histologic autofluorescence than Beckman classification.
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