禽致病性大肠杆菌(APEC),一种广泛存在的细菌,每年给家禽业造成严重的经济损失,由于受污染的零售禽肉和鸡蛋,它对人类健康构成威胁。最近,已经证明长链非编码RNA在调节基因表达和动物免疫应答中起着重要作用。本研究旨在系统探索新型长基因间非编码转录本的功能,lincRNA-73240,在APEC感染后。生物信息学分析表明,lincRNA-73240没有编码能力,二级结构相对稳定,有多个发夹环。此外,RT-qPCR结果显示lincRNA-73240在肺部高表达,心,肝脏,脾,脾盲肠扁桃体,胸腺,回肠,法布里修斯的法萨,Harderian腺体,与大脑相比,肌肉。此外,lincRNA-73240的过表达可以促进炎症的表达水平,凋亡,自噬,和氧化应激相关基因,以及活性氧(ROS)的产生,丙二醛(MDA),和一氧化氮(NO)在APEC感染时,导致细胞损伤和凋亡。这些发现为研究鸡lincRNA-73240的生物学功能奠定了基础,为进一步研究鸡免疫应答的分子机制提供了理论依据。
Avian pathogenic E. coli (APEC), a widespread bacterium, results in serious economic losses to the poultry industry annually, and it poses a threat to human health due to the contaminated retail poultry meat and eggs. Recently, it has been demonstrated that long non-coding RNAs played important roles in regulating gene expression and the animal immune response. This study aimed to systematically explore the function of the novel long intergenic non-coding transcript, lincRNA-73240, upon APEC infection. A bioinformatics analysis indicated that lincRNA-73240 had no coding ability and a relative stable secondary structure with multiple hairpin rings. Moreover, the RT-qPCR results showed that lincRNA-73240 was highly expressed in lungs, heart, liver, spleen, cecum tonsils, thymus, ileum, bursa of Fabricius, harderian gland, and muscles in comparison to the cerebrum. Additionally, overexpression of lincRNA-73240 can promote the expression levels of inflammation, apoptosis, autophagy, and oxidative stress-related genes, as well as the production of reactive oxygen species (ROS), malondialdehyde (MDA), and nitric oxide (NO) upon APEC infection, which lead to cellular injury and apoptosis. These findings collectively establish a foundation for the study of the biological function of chicken lincRNA-73240 and provide a theoretical basis for further research on the molecular mechanisms of the chicken immune response.