■果蝇是光滑的,低维护和安全的模型生物,它们可以毫不费力地用于生命科学的不同领域,如基因组学,生物技术,遗传学,疾病模型,以及基于Wolbachia的方法来对抗媒介和它们传播的病原体。
■在伊朗25个地区(14个省)收集了果蝇标本,并通过基于线粒体COI条形码区序列同源性的分子分析证明了其形态识别。提供了D.melanogaster实验室饲养的基本信息和具体要求。
■在25个地区中的23个地区发现了果蝇菌落。此外,在这项研究中报告了与D.melanogaster重合的五个相关物种,包括D.ananassae/D。parapallidosa,D.hydei,D.repleta,Zaprionusindianus(双翅目:果蝇科),和巨大的scalaris(双翅目:phoridae)。这里描述了伊朗D.melanogaster分子特征及其饲养技术。完整的生命周期,从(鸡蛋到成人),在25°C下大约需要8天。一些生物学点已经呈现为突出捕获,饲养,培养,并提出了胚胎收集以及原始的识别和雌雄之间的隔离。已经提供了培养基的配方和各种成分的量。
■这是关于D.repleta和D.ananassae/D的第一份报告。该国的parapallidosa物种。这项研究的结果提供了高效和有效的饲养程序,这对于小规模促进昆虫学研究和大规模使用合理的病媒控制管理(如疾病模型或登革热控制)都是必需的。
UNASSIGNED: Drosophila melanogaster flies are smooth, low upkeep and safe model organisms, they can be effortlessly used in different fields of life sciences like genomics, biotechnology, genetics, disease model, and Wolbachia-based approaches to fight vectors and the pathogens they transmit.
UNASSIGNED: Fruit fly specimens were collected in 25 districts (14 provinces) of Iran and their morphological recognition was proven by molecular analysis based on sequence homology of mitochondrial COI barcode region. Essential information and specific requirements were provided for laboratory rearing of D. melanogaster.
UNASSIGNED: Drosophila melanogaster colonies were found in 23 out of 25 districts. Also, five related species coincident with D. melanogaster were reported in this study including D. ananassae/D. parapallidosa, D. hydei, D. repleta, Zaprionus indianus (Diptera: Drosophilidae), and Megaselia scalaris (Diptera: Phoridae). The Iranian D. melanogaster molecular signature and their rearing techniques have been described here. The complete life cycle, from (egg to adult), takes approximately 8 days at 25 °C. Some biological points have been presented with highlighting capturing, rearing, culturing, and embryo collection along with primitive recognition and segregation between females and males have been presented. A recipe for culture media and the quantity of various ingredients have been provided.
UNASSIGNED: This is the first report on the D. repleta and D. ananassae/D. parapallidosa species for the country. Results of this study provide efficient and effective rearing procedures which are requirement for both small-scale for facilitating entomological research and large-scale use in justifiable vector control management such as disease model or Dengue control.