目的:目的:在孕妇缺铁性贫血的背景下,采用化学发光和组织化学两种研究方法,建立慢性绒毛膜羊膜炎胎盘绒毛膜板内皮细胞自由基过程的特征。
方法:材料和方法:研究了妊娠37-40周产妇的82个胎盘。包括,为了比较,胎盘生理性妊娠和缺铁性贫血孕妇胎盘无炎症的观察。表中给出了特定研究组的观察次数。为了实现本研究的目标并解决本研究中设定的任务,进行了以下组织化学,化学发光,材料加工的形态计量学和统计学方法。
结果:结果:在妊娠贫血背景下发生绒毛膜羊膜炎,根据MikelCalvo的溴酚蓝方法中的R/B比(R/B-蛋白质的氨基(蓝色)和羧基(红色)之间的比)为1.56±0.021,氮氧化物的化学发光指标为133±4.5,使用A.Yasuma和T.Ichikawa的方法进行组织化学染色的相对光密度单位为-0.224±0.0015。
结论:结论:慢性绒毛膜羊膜炎,氮氧化物的辉光强度,R/B比率的平均指标,与生理性妊娠胎盘和孕妇贫血相比,蛋白质游离氨基的组织化学染色光密度增加。孕妇贫血导致氮氧化物发光强度增加,R/B比率的平均值,以及与无贫血的炎症胎盘相比,蛋白质游离氨基的组织化学染色的光密度。慢性绒毛膜羊膜炎合并贫血的形态特征形成的关键因素是自由基过程的加剧,这反映在炎症中心的氮氧化物浓度增加,随着蛋白质氧化修饰过程的加剧,随后是有限蛋白水解过程的活性增加。
OBJECTIVE: Aim: To establish the features of free radical processes in the endotheliocytes of the chorionic plate of the placenta in chronic chorioamnionitis against the background of iron deficiency anemia of pregnant women using both chemiluminescent and histochemical methods of research.
METHODS: Materials and Methods: 82 placentas from parturients at 37 - 40 weeks of gestation were studied. Including, for comparison, the placenta during physiological pregnancy and the observation of iron deficiency anemia of pregnant women without inflammation of the placenta. The number of observations in specific study groups is given in the tables. To achieve the objective and solve the tasks set in this study, there were carried out the following histochemical, chemiluminescent, morphometric and statistical methods of material processing.
RESULTS: Results: In case of chorionamnionitis against the background of anemia in pregnancy, the R/B ratio (R/B - ratio between amino- (blue) and carboxyl (red) groups of proteins)) in the method with bromophenol blue according to Mikel Calvo was 1.56±0.021, indicators of chemiluminescence of nitroperoxides were 133±4.5, relative optical density units of histochemical staining using the method according to A. Yasuma and T. Ichikawa was - 0.224±0.0015.
CONCLUSIONS: Conclusions: With chronic chorioamnionitis, the intensity of the glow of nitroperoxides, the average indicators of the R/B ratio, and the optical density of histochemical staining for free amino groups of proteins are increased compared to placentas of physiological pregnancy and anemia of pregnant women. Comorbid i anemia of pregnant women causes increasing of the intensity of the glow of nitroperoxides, the average values of the R/B ratio, and the optical density of histochemical staining for free amino groups of proteins comparing to placentas with inflammation without anemia. The key factor in the formation of morphological features of chronic chorioamnionitis with comorbid anemia is the intensification of free radical processes, which is reflected by the increase in the concentration of nitroperoxides in the center of inflammation, with the subsequent intensification of the processes of oxidative modification of proteins, which is followed by the increasing activity of the processes of limited proteolysis.