食品安全和食源性感染是全球关注的主要主题。乳制品被认为是这些感染的重要来源。
■进行本研究以确定葡萄球菌的发生和基因型分离株。从Al-Diwaniyah市的牛奶样本中回收,伊拉克。
■当前的研究包括从当前城市的当地商店购买的50个牛奶样品的收集。对这些样品进行细菌培养和生化测试。稍后,该生长用于提取暴露于PCR和部分基因测序的基因组DNA,两者都靶向特定遗传片段的16SrRNA基因。
■PCR结果证明了金黄色葡萄球菌(SAD11,SAD12,SAD13,SAD14和SAD15)的五个遗传簇的遗传片段的扩增,表皮葡萄球菌(SED1、SED2、SED3、SED4和SED5)和中间葡萄球菌(SID1,SID2,SID3,SID4和SID5)。将PCR产物送去测序,报告目前分离株的基因含量与全球分离株相似,为95.34%至97.59%,96.21%到97.57%,和96.09%到97.88%,分别,身份。
■目前的研究结果表明,金黄色葡萄球菌的分离株之间存在高度的遗传变异,表皮葡萄球菌,和从牛奶样本中回收的中间美国,这些基因型在与人类和动物相关的不同感染环境中发现,这可能对人类和动物构成高风险。
UNASSIGNED: Food safety and food-borne infections are major subjects of global interest. Dairy products are considered as important source for these infections.
UNASSIGNED: The present study was conducted to identify the occurrence and to genotype isolates of Staphylococcus spp. recovered from milk samples in Al-Diwaniyah City, Iraq.
UNASSIGNED: The current study included the collection of 50 milk samples purchased from local stores in the current city. These samples were subjected to bacterial cultivation and biochemical tests. Later, the growth was used to extract the genomic DNA that was exposed to PCR and partial gene sequencing both targeted the 16S rRNA gene at a specific genetic piece.
UNASSIGNED: The PCR results demonstrated the amplification of the genetic fragment of five genetic clusters for each of Staphylococcus aureus (SAD11, SAD12, SAD13, SAD14, and SAD15), Staphylococcus epidermidis (SED1, SED2, SED3, SED4, and SED5), and Staphylococcus intermedius (SID1, SID2, SID3, SID4, and SID5). The PCR products were sent out to sequencing and reported that the current isolates were similar in their genetic content with global isolates at 95.34% to 97.59%, 96.21% to 97.57%, and 96.09% to 97.88%, respectively, of identity.
UNASSIGNED: The present findings show high genetic variations among isolates of S. aureus, S. epidermidis, and S. intermedius recovered from milk samples, and these genotypes are found in different infection settings related to humans and animals, which may pose high risks to humans and animals.