一种新型罗丹明基多离子荧光传感器,RGN,设计和合成用于乙醇和水系统中汞离子(Hg2+)的高选择性检测,以及三价阳离子(Fe3+,Al3+,和Cr3)在乙腈和水系统中使用两步席夫碱反应方法。核磁滴定实验和理论计算表明,该传感器通过内酰胺开环诱导的荧光导通现象实现了对上述金属离子的检测。密度泛函理论(DFT)计算结果表明HOMO-LUMO能隙减小,偶极矩增加,表明传感器与相应的金属离子有效配位形成更稳定的络合物,从而实现检测目标。此外,荧光开启传感器RGN表现出相对较低的检测限,具有Fe3+的检测限(LOD),Al3+,Cr3+,Hg2+为10.20nM,14.66nM,58.78nM,和73.33nM,分别。最后,传感器RGN在环境水样中的实际应用,L929细胞,斑马鱼被证明,表明其检测和跟踪Fe3+的潜力,Al3+,Cr3+,和环境样品和生物系统中的Hg2+,在重金属离子引起的疾病的诊断和治疗中的生物医学应用前景。
A novel rhodamine-based multi-ion fluorescent sensor, RGN, was designed and synthesized for the highly selective detection of mercury ions (Hg2+) in ethanol and water systems, as well as trivalent cations (Fe3+, Al3+, and Cr3+) in acetonitrile and water systems using a two-step Schiff base reaction method. Nuclear magnetic titration experiments and theoretical calculations demonstrated that the sensor achieved the detection of the aforementioned metal ions through the fluorescence turn-on phenomenon induced by lactam ring-opening. Density functional theory (DFT) calculation results showed decreased HOMO-LUMO energy gaps and increased dipole moments, indicating the effective coordination of the sensor with the corresponding metal ions to form more stable complexes, thereby achieving detection objectives. Furthermore, the fluorescence turn-on sensor RGN exhibited relatively low detection limits, with limits of detection (LOD) for Fe3+, Al3+, Cr3+, and Hg2+ being 10.20 nM, 14.66 nM, 58.78 nM, and 73.33 nM, respectively. Finally, practical applications of sensor RGN in environmental water samples, L929 cells, and zebrafish were demonstrated, indicating its potential for detecting and tracking Fe3+, Al3+, Cr3+, and Hg2+ in environmental samples and biological systems, with prospects for biomedical applications in the diagnosis and treatment of heavy metal ion-induced diseases.