硫化氢(H2S)的平衡态,由溶酶体代谢物产生的气体信号分子,体内对细胞功能至关重要。H2S浓度的异常波动会干扰溶酶体的正常功能,与多种疾病的发病机制密切相关。鉴于此,开发了一种基于1,3,5-三芳基吡唑啉的新型荧光探针Lyso-DPP,用于通过使用亲水性吗啉部分作为溶酶体靶向单位来精确检测溶酶体中的H2S,和2,4-二硝基苯甲醚作为荧光猝灭和H2S响应单元。该探针巧妙地结合了合成简单的优点,灵敏的蓝色荧光开启,具有检测限,LOD,97.3nM,稳定性好,和快速的响应时间(10分钟),这使得Lyso-DPP成功地以试纸的形式便携式监测肉类新鲜度。此外,Lyso-DPP探针的优异的生物相容性和精确的靶向能力使其在溶酶体中H2S的监测中表现良好,活细胞,还有斑马鱼.这项工作不仅为食品质量控制提供了新的技术工具,而且为H2S相关疾病的早期诊断和治疗提供了新思路。
The equilibrium state of hydrogen sulfide (H2S), a gaseous signaling molecule produced by lysosomal metabolites, in vivo is crucial for cellular function. Abnormal fluctuations in H2S concentration can interfere with the normal function of lysosomes, which has been closely linked to the pathogenesis of a variety of diseases. In view of this, a novel fluorescent probe Lyso-DPP based on 1,3,5-triarylpyrazolines was developed for the precise detection of H2S in lysosomes by using the hydrophilic morpholine moiety as a lysosomal targeting unit, and 2,4-dinitroanisole as a fluorescence-quenching and H2S-responsive unit. The probe cleverly combines the advantages of simple synthesis, sensitive blue fluorescence turn-on with a limit of detection, LOD, of 97.3 nM, good stability, and fast response time (10 min), which makes Lyso-DPP successful in portable monitoring of meat freshness in the form of test strips. Moreover, the excellent biocompatibility and precise targeting capability of the probe Lyso-DPP make it perform well in the monitoring of H2S in lysosomes, living cells, and zebrafish. This work not only provides new technical tools for food quality control but also paves up new ideas for early diagnosis and treatment of H2S-related diseases.