飞行是一个复杂的生理过程,需要精确协调肌肉收缩。昆虫飞行中的关键蛋白质是飞食素,在飞行肌肉中起着不可或缺的作用。这项研究试图通过表征所涉及的分子成分来评估社交黄蜂的飞行能力。我们的研究集中在基底Vespa,最危险的大黄蜂物种之一,利用PCR获得Flightin蛋白的部分cDNA序列。然后,我们采用系统发育和序列分析来了解与飞行相关的适应中的这种蛋白质。cDNA具有1189个碱基对序列,包括编码150个氨基酸的开放阅读框(453bp)。使用在线工具分析推导的氨基酸序列显示分子量为18.05kDa,5.84的等电点,四个功能位点模式,没有跨膜拓扑结构.我们基于38个物种构建了flightin的系统发育树。我们的分析表明,巴氏弧菌与曼陀林弧菌关系最密切;这种排列与它们相似的攻击行为是一致的,但它们的进化关系,基于线粒体序列,呈现对比。关于基底弧菌flightin基因的这些初步发现为未来的功能研究奠定了基础,以阐明其在飞行适应中的特定作用,并探索其作为害虫管理策略目标的潜力。
Flight is a complex physiological process requiring precise coordination of muscular contraction. A key protein in insect flight is
flightin, which plays an integral role in the flight muscles. This research sought to evaluate the flight competence of the social wasp V. basalis by characterizing the molecular components involved. Our study focused on Vespa basalis, one of the most dangerous hornet species, utilizing PCR to obtain a partial cDNA sequence of the
flightin protein. We then employed phylogenetic and sequence analysis to gain insights into this protein in flight-related adaptations. The cDNA has an 1189-base pair sequence including an open reading frame (453 bp) encoding 150 amino acids. Analyzing the deduced amino acid sequence using an online tool revealed a molecular weight of 18.05 kDa, an isoelectric point of 5.84, four functional site patterns, and no transmembrane topology. We constructed a phylogenetic tree of
flightin based on 38 species. Our analysis indicated that V. basalis is most closely related to V. mandarinia; this alignment is consistent with their similar aggressive behavior, but their evolutionary relationship, based on mitochondrial sequences, presents a contrast. These initial findings on the
flightin gene in V. basalis lay the groundwork for future functional studies to elucidate its specific role in flight adaptations and explore its potential as a target for pest management strategies.