在目前的工作中,我们已经研究了来自保加利亚的小麦的多酚成分。多酚用不同极性的溶剂分馏(正己烷,氯仿,乙酸乙酯,和正丁醇)。通过HPLC-PDA和UHPLC-MS分析级分。乙酸乙酯部分含有槲皮素的单-和二-糖苷,山奈酚的二糖苷,以及异鼠李素和组织蛋白单糖苷和黄羊甙。我们在丁醇部分中发现槲皮素三糖苷。乙酸乙酯和丁醇级分含有168.82mg/gExtr和67.21mg/gExtr槲皮素糖苷,分别。C.botrys中多酚复合物的主要成分是6-甲氧基黄酮(355.47mg/gExtr),在氯仿部分中发现。黄酮类果胶素,去甲基金黄素,和异辛酮,和槲皮素的糖苷(三糖苷,酰基糖苷),山奈酚,异鼠李素,hispidiulin,和黄菊,这是第一次在荆棘中发现和报道。我们使用体外方法评估抗氧化应激的生物活性(过氧化氢清除活性(HPSA)和羟自由基清除活性(HRSA)),硝化应激(一氧化氮清除活性(NOSA)),抗炎活性(IAD抑制),和抗胰蛋白酶活性(ATA)。槲皮素单糖苷和二糖苷表现出更高的HPSA和HRSA(IC50=39.18,105.03µg/mL),而6-甲氧基黄酮具有更大的NOSA(IC50=146.59µg/mL)。相同的成分显示最高的ATA(IC50范围为116.23至202.44µg/mL)。
In the present work, we have investigated the polyphenolic composition of Chenopodium botrys from Bulgaria. The polyphenols were fractionated with solvents of varying polarity (n-hexane, chloroform, ethyl acetate, and n-butanol). The fractions were analyzed by HPLC-PDA and UHPLC-MS. The ethyl acetate fraction contained mono- and di-glycosides of quercetin, di-glycosides of kaempferol, and isorhamnetin and monoglycosides of hispidulin and jaceosidine. We found quercetin triglycosides in the butanol fraction. The ethyl acetate and butanol fractions contained 168.82 mg/g Extr and 67.21 mg/g Extr of quercetin glycosides, respectively. The main components of the polyphenolic complex in C. botrys were 6-methoxyflavones (355.47 mg/g Extr), which were found in the chloroform fraction. The flavonoids pectolinarigenin, demethylnobiletin, and isosinensetin, and the glycosides of quercetin (triglycosides, acylglycosides), kaempferol, isorhamnetin, hispidiulin, and jaceosidine, were discovered and reported in Chenopodium botrys for the first time. We used in vitro methods to assess the biological activity against oxidative stress (hydrogen peroxide scavenging activity (HPSA) and hydroxyl radical scavenging activity (HRSA)), nitrosative stress (nitric oxide scavenging activity (NOSA)), anti-inflammatory activity (IAD inhibition), and anti-tryptic activity (ATA). Quercetin mono- and di-glycosides exhibited greater HPSA and HRSA (IC50 = 39.18, 105.03 µg/mL), while 6-methoxyflavones had a greater NOSA (IC50 = 146.59 µg/mL). The same components showed the highest ATA (IC50 ranging from 116.23 to 202.44 µg/mL).