鱼类传播的人畜共患吸虫Haplorchispumilio(Looss,1896)是根据形态学在巴西首次报道的,实验和分子研究。胸膜夜囊尾蚴是从Ceaá-Mirim市收集的侵入性蜗牛Melanoidestaterculata中出现的,北里奥格兰德州,巴西东北地区,2018年11月,用于孔雀鱼的实验性感染,网状Poecilia。从这些鱼类的尾肌肉组织中提取了cer虫,并用于感染小鼠。在小鼠小肠中回收的成年寄生虫在形态学上被鉴定为H.pumilio。获得了H.pumilio的巴西和秘鲁分离株的分子序列,并与GenBank提供的数据进行了比较。对核基因28S(1219bp)和ITS-2(290bp)片段的分析显示,南美和亚洲分离株之间的相似性为98.48-100%。此外,从巴西(797bp)和秘鲁(646pb)分离株获得的线粒体基因cox-1的新序列与该物种的墨西哥分离株100%相似,与泰国分离株97.54%相似。这一发现揭示了巴西人类单株病发生的可能性,特别是因为生鱼菜在这个国家越来越受欢迎。
The fishborne zoonotic trematode Haplorchis pumilio (Looss, 1896) is here reported for the first time in Brazil based on morphological, experimental and molecular studies. Pleurolophocercous cercariae emerged from the invasive snail Melanoides tuberculata collected in the municipality of Ceará-Mirim, state of Rio Grande do Norte, Northeast region of Brazil, in November 2018, were used for experimental infection of guppies, Poecilia reticulata. Metacercariae were extracted from the caudal musculature of these fishes and were used to infect mice. Adult parasites recovered in the small intestine of the mice were morphologically identified as H. pumilio. Molecular sequences were obtained for the Brazilian and Peruvian isolates of H. pumilio, and were compared with data available in GenBank. Analyses of fragments of the nuclear genes 28S (1219 bp) and ITS-2 (290 bp) revealed 98.48-100% similarity between the South American and Asian isolates of H. pumilio. Moreover, new sequences of the mitochondrial gene cox-1 obtained for the Brazilian (797 bp) and Peruvian (646 pb) isolates were 100% similar to a Mexican isolate of this species and 97.54% similar to an isolate from Thailand. This finding reveals the potential for occurrence of human haplorchiasis in Brazil, especially because of the increasing popularity of raw fish dishes in the country.