2型糖尿病(T2D)与女性不孕症(FI)有关。然而,我们对分子标志和潜在机制的理解仍然难以捉摸。这篇研究文章旨在寻找集线器基因,通路,转录因子,和miRNA参与。对于这项研究,像细胞景观这样的软件,字符串,Enrichr,FFL回路,等。,被利用。本研究使用差异表达基因(DEGs)来鉴定多个生物学靶标,以了解T2D与女性不孕症(FI)之间的关联。在T2D和FI之间,我们发现3869个差异表达基因。我们还分析了不同的途径,如甲状腺激素信号通路,AGE-RAGE信号通路在糖尿病并发症和泛素介导的蛋白水解中的作用.此外,hub基因MED17,PRKCG,THRA,FOXO1,NCOA2,PLCG2,COL1A1,CXCL8,PRPF19,ANAPC5,UBE2I,已确定XIAP和KEAP1。此外,这些hub基因被用于鉴定T2D相关女性不育症特异性miRNA-mRNA调控网络.在FFL研究(前馈回路)中,转录因子(SP1,NFKB1,RELA和FOX01),miRNA(has-mir-7-5p,has-let-7a-5p,hsa-mir-16-5p,hsa-mir-155-5p,has-mir-122-5p,has-let-7b-5p,has-mir-124-3p,has-mir-34a-5p,has-mir-130a-3p,has-let-7i-5p,和hsa-mir-27a-3p)和六个基因(XIAP,THRA,13个关键基因中的NCOA2,MED17,FOXO1和COL1A1)被认为是调节剂和抑制剂。我们的分析表明,这些基因可以作为与2型糖尿病相关的女性不孕症的重要生物标志物。通过候选基因的优先排序。这项研究使我们深入了解T2D相关FI的分子和细胞机制。这一发现有助于开发新的治疗方法,并将提高疗效并减少治疗的副作用。这项研究需要对主要目标进行进一步的实验研究。
Type 2 diabetes mellitus (T2D) has been linked with female infertility (FI). Nevertheless, our understanding of the molecular hallmarks and underlying mechanisms remains elusive. This research article aimed to find the hub genes, pathways, transcription factors, and miRNA involved. For this study, softwares like cytoscape, string, Enrichr, FFL loop, etc., were utilized. This research article employed differentially expressed genes (DEGs) to identify multiple biological targets to understand the association between T2D and female infertility (FI). Between T2D and FI, we found 3869 differentially expressed genes. We have also analyzed different pathways like thyroid hormone signaling pathways, AGE-RAGE signaling pathways in diabetic complications and ubiquitin-mediated proteolysis through pathway analysis. Moreover, hub genes MED17, PRKCG, THRA, FOXO1, NCOA2, PLCG2, COL1A1, CXCL8, PRPF19, ANAPC5, UBE2I, XIAP and KEAP1 have been identified. Additionally, these hub genes were subjected to identify the miRNA-mRNA regulation network specific to T2D-associated female infertility. In the FFL study (Feed Forward Loop), transcription factor (SP1, NFKB1, RELA and FOX01), miRNA (has-mir-7-5p, has-let-7a-5p, hsa-mir-16-5p, hsa-mir-155-5p, has-mir-122-5p, has-let-7b-5p, has-mir-124-3p, has-mir-34a-5p, has-mir-130a-3p, has-let-7i-5p, and hsa-mir-27a-3p) and six genes (XIAP, THRA, NCOA2, MED17, FOXO1, and COL1A1) among the thirteen key genes were recognized as regulator and inhibitor. Our analysis reveals that these genes can serve as a significant biomarker for female infertility linked with Type 2 Diabetes, through the prioritization of candidate genes. This study gives us insight into the molecular and cellular mechanism of T2D-associated FI. This finding helps in developing novel therapeutic approaches and will improve efficacy and reduce side effects of the treatment. This research requires further experimental investigation of the principal targets.