已经发现甲醛降低饲料和成分基质中的病毒浓度。需要继续研究以确定这些基质中不同病毒的适当包含水平和应用时间。目的是评估猪流行性腹泻病毒(PEDV)接种前或接种后甲醛的不同包合水平,2型猪繁殖与呼吸综合征病毒(PRRSV),和塞内卡谷病毒1(SVV1)来完成饲料或豆粕。实验是在2×2阶乘中设计的,使用基于甲醛的产品(Termin-8,AnitoxCorporation,劳伦斯维尔,GA)在病毒接种之前(接种前)或接种后(接种后)以2或3kg/MT施用。在第0天,将相应基质的样品称重为50g等分试样并添加到500mL瓶中。将甲醛以各自的包含水平应用于接种前样品,并将50μL的每种病毒添加到接种后样品中。将所有瓶摇动并使其在室温下静置24小时。在第1天,将病毒添加到接种前样品中并将甲醛添加到接种后瓶中。立即处理一半样品(0小时),另一半在室温下再孵育24小时。处理样品并通过三重PCR分析等分试样。在完全饲料中,PEDV在0h和SVV1和PEDV在24h观察到施用时间×包合水平相互作用,其中与阳性对照相比,在任一包含水平的接种后样品中检测到较少的病毒RNA(P<0.05)。在豆粕中,在0h的PEDV和PRRSV中观察到相同的相互作用,在24h的SVV1和PEDV中观察到相同的相互作用,与接种前对应物和对照相比,在接种后样品中观察到较少的可检测RNA(P<0.05)。总的来说,在每个基质中注意到应用时间效应,与接种前样品和对照相比,接种后样品在0h检测到较少的RNA(P<0.05)。在24小时,接种前和接种后样本的RNA检测率均低于对照(P<0.05).总的来说,甲醛可以立即减少污染的完整饲料和豆粕中可检测到的RNA,随着缓解剂接触时间的增加,观察到更大的减少。
Formaldehyde has been found to decrease virus concentrations in
feed and ingredient matrices. Continued research is needed to identify the appropriate inclusion levels and application time for different viruses in these matrices. The objective was to evaluate different inclusion levels of formaldehyde when applied either pre- or postinoculation of porcine epidemic diarrhea virus (PEDV), type 2 porcine reproductive and respiratory syndrome virus (PRRSV), and Seneca Valley virus 1 (SVV1) to complete
feed or soybean meal. The experiment was designed in a 2 × 2 factorial with a formaldehyde-based product (Termin-8, Anitox Corporation, Lawrenceville, GA) applied either before virus inoculation (preinoculation) or after inoculation (postinoculation) at either a 2 or 3 kg/MT. On day 0, samples of the respective matrices were weighed in 50 g aliquots and added to 500 mL bottles. Formaldehyde was applied to the preinoculation samples at the respective inclusion levels and 50 µL of each virus were added to the postinoculation samples. All bottles were shaken and allowed to sit at room temperature for 24 h. On day 1, virus was added to the preinoculation samples and formaldehyde was added to the postinoculation bottles. Half of the samples were immediately processed (0 h) and the other half were incubated at room temperature for an additional 24 h. Samples were processed and aliquots were analyzed via triplex PCR. An application time × inclusion level interaction was observed for PEDV at 0 h and SVV1 and PEDV at 24 h in complete
feed, where less viral RNA (P < 0.05) was detected in the postinoculation samples at either inclusion level as compared to the positive controls. In soybean meal, the same interaction was observed in PEDV and PRRSV at 0 h and SVV1 and PEDV at 24 h with less detectable RNA observed (P < 0.05) in the postinoculation samples regardless of inclusion level than the preinoculation counterparts and the controls. Overall, an application time effect was noticed in each matrix where less RNA was detected in the postinoculation samples at 0 h (P < 0.05) compared to the preinoculation samples and the control, and at 24 h, both the pre- and postinoculation samples had less detectable RNA (P < 0.05) than the control. Overall, formaldehyde can reduce detectable RNA immediately in contaminated complete
feed and soybean meal, with greater decreases observed as mitigant contact time increases.