Selective and sensitive dopamine (DPA) sensor was developed using hydrothermally prepared functionalized multi-walled carbon nanotube-coated bismuth molybdate (f-MWCNT@BMO). The f-MWCNT@BMO-reinforced electrode exhibited an outstanding electrocatalytic activity towards DPA oxidation. The nanocomposite-reinforced electrode displayed a rapid response towards DPA sensing and possessed the minimized potential of (Epa + 0.285 V vs Ag/AgCl) in 0.1 M phosphate buffer (PB). The electrochemical results of prepared sensors were analyzed using the differential pulse voltammetry method (DPV). As a result, the f-MWCNT@BMO-reinforced electrode exhibited a widelinear range of 10 nM - 814 μM with a very low detection limit of 3.4 nM towards DPA oxidation. The developed sensor shows excellent selectivity in presence of similar functional group biomolecules. The detection of DPA in real samples was evaluated in human serum, as the results of the proposed sensor possessed good recoveries.