背景:全身施用的抗生素被认为在负压伤口治疗(NPWT)期间更有效地穿透伤口。为了检验该假设,在血清和伤口渗出物中定量总的和游离的抗生素浓度。
方法:开发并验证了UHPLC-MS/MS方法,用于测定头孢他啶,头孢吡肟,头孢噻肟,头孢呋辛,头孢唑啉,美罗培南,苯唑西林,哌拉西林和他唑巴坦,克林霉素,环丙沙星,磺胺甲恶唑/甲氧苄啶(复方新诺明),庆大霉素,万古霉素,和利奈唑胺.使用MilliporeMicrocon-30kda离心过滤单元通过超滤获得未结合的抗生素级分。在1.7μmAcquityUPLCBEHC182.1×100mm色谱柱上进行梯度洗脱分析。
结果:对血清进行验证,分泌物和游离部分。对于所有矩阵,满足关于线性的要求,精度,准确度,定量极限,和矩阵效应。变异系数在1.2-13.6%的范围内。回收率为87.6-115.6%,分别。到目前为止,在29种抗生素应用中,包括万古霉素,克林霉素,环丙沙星,苯唑西林,头孢吡肟,头孢噻肟,复方新诺明,还有庆大霉素,血清和渗出物中的总抗生素浓度和游离抗生素浓度相关。
结论:该方法可以准确定量16种抗生素的总浓度和游离浓度。比较血清和渗出物之间的浓度比可以监测接受NPWT的患者的个体抗生素渗透能力。
BACKGROUND: Systemically administered antibiotics are thought to penetrate the wounds more effectively during negative pressure wound therapy (NPWT).To test this hypothesis total and free antibiotic concentrations were quantified in serum and wound
exudate.
METHODS: UHPLC-MS/MS methods were developed and validated for the determination of ceftazidime, cefepime, cefotaxime, cefuroxime, cefazolin, meropenem, oxacillin, piperacillin with tazobactam, clindamycin, ciprofloxacin, sulfamethoxazole/trimethoprim (cotrimoxazole), gentamicin, vancomycin, and linezolid. The unbound antibiotic fraction was obtained by ultrafiltration using a Millipore Microcon-30kda Centrifugal Filter Unit. Analysis was performed on a 1.7-µm Acquity UPLC BEH C18 2.1 × 100-mm column with a gradient elution.
RESULTS: The validation was performed for serum, exudates and free fractions. For all matrices, requirements were met regarding linearity, precision, accuracy, limit of quantitation, and matrix effect. The coefficient of variation was in the range of 1.2-13.6%.and the recovery 87.6-115.6%, respectively. Among the 29 applications of antibiotics thus far, including vancomycin, clindamycin, ciprofloxacin, oxacillin, cefepime, cefotaxime, cotrimoxazole, and gentamicin, total and free antibiotic concentrations in serum and
exudate were correlated.
CONCLUSIONS: This method can accurately quantify the total and free concentrations of 16 antibiotics. Comparison of concentration ratios between serum and exudates allows for monitoring individual antibiotics\' penetration capacity in patients receiving NPWT.