松果是一种花黄色的灌木,高达两米,智利阿塔卡马地区的特有种。该物种在低海拔等特殊环境条件下生长,干旱地区,和直接暴露在阳光下的栖息地。在本研究中,乙醇提取物是从辣椒的果实中获得的,然后在极性增加的溶剂中分配以获得五个馏分:己烷(HF),二氯甲烷(DF),乙酸乙酯(AF),和残余水分数(QF)。提取物的抗氧化活性通过使用DPPH,ABTS,和FRAP方法。结果表明,辣椒的抗氧化能力高于在类似环境中生长的其他植物的抗氧化能力。这种作用归因于在辣椒中发现的最高含量的类黄酮和总酚。另一方面,在不同提取物级分存在下评估乳腺癌细胞系(MCF-7)和非肿瘤细胞系(MCF-10A)的细胞活力。结果表明,己烷部分(HF)对两种细胞系均表现出最高的细胞毒性(IC50值等于35和45µg/mL),而二氯甲烷部分(DF)是最具选择性的。二氯甲烷馏分(DF)的GC-MS分析显示脂肪酸的存在,糖,糖和多元醇作为主要成分。
Pintoa chilensis is a shrub with yellow flowers that reach up to two meters high, endemic of the Atacama Region in Chile. This species grows under special environmental conditions such as low altitude, arid areas, and directly sun-exposed habitats. In the present study, ethanolic extract was obtained from fruits of P. chilensis, and then partitioned in solvents of increasing polarity to obtain five fractions: hexane (HF), dichloromethane (DF), ethyl acetate (AF), and the residual water fraction (QF). The antioxidant activity of extracts was evaluated by using the DPPH, ABTS, and FRAP methods. The results show that the antioxidant capacity of P. chilensis is higher than that reported for other plants growing in similar environments. This effect is attributed to the highest content of flavonoids and total phenols found in P. chilensis. On the other hand, the cell viability of a breast cancer cell line (MCF-7) and a non-tumor cell line (MCF-10A) was assessed in the presence of different extract fractions. The results indicate that the hexane fraction (HF) exhibits the highest cytotoxicity on both cell lines (IC50 values equal to 35 and 45 µg/mL), whereas the dichloromethane fraction (DF) is the most selective one. The GC-MS analysis of the dichloromethane fraction (DF) shows the presence of fatty acids, sugars, and polyols as major components.