间充质干细胞(MSC)在人和兽医临床应用中的使用已成为越来越重要的主题,因为它们在免疫调节和再生过程中的作用。MSCs在马医学中尤其相关,因为它们可能具有治疗普遍存在的肌肉骨骼疾病的能力。除了其他条件。然而,最近的证据表明,由MSCs分泌的成分,特别是细胞外囊泡(EV),负责这些属性。电动汽车含有蛋白质和核酸,在细胞间通讯中具有积极作用,可用作治疗。然而,因为马兽医学与电动汽车的交叉仍然是一个相对较新的领域,有一种需求来识别生物标志物,可以辨别和丰富治疗性电动汽车,提高其临床疗效。在这项研究中,我们鉴定并表征了84个miRNAs,在细胞和EV受试者中参与免疫调节的三个马供体之间。我们发现了不同组的共享miRNA,像miR-21-5p和miR-451a,捐赠者之间的丰富和丰富的电动汽车,分别。通过定位和比较MSC-EVmiRNA表达,我们发现了许多与马临床应用相关的免疫调节和组织再生过程相关的途径。因此,本文重点介绍的miRNA可作为有价值的生物标志物,用于筛选用于潜在马治疗的MSC来源的EV.
The use of mesenchymal stem cells (MSCs) in human and veterinary clinical applications has become a subject of increasing importance due to their roles in immunomodulation and regenerative processes. MSCs are especially relevant in equine medicine because they may have the ability to treat prevalent musculoskeletal disorders, among other conditions. However, recent evidence suggests that the components secreted by MSCs, particularly extracellular vesicles (EVs), are responsible for these properties. EVs contain proteins and nucleic acids, which possess an active role in intercellular communication and can be used as therapeutics. However, because the intersection of equine veterinary medicine with EVs remains a relatively new field, there is a demand to identify biomarkers that can discern and enrich for therapeutic EVs, progressing their clinical efficacy. In this study, we identified and characterized 84 miRNAs, between three equine donors involved in immunomodulation in cell and EV subjects. We discovered distinct groups of shared miRNAs, like miR-21-5p and miR-451a, that are abundant and enriched between the donors\' EVs, respectively. By mapping and comparing the MSC-EV miRNA expression, we discovered many pathways that are involved in immunomodulation and tissue regenerative processes related to equine clinical applications. Therefore, the miRNAs highlighted in this article can be used as valuable biomarkers for screening MSC-derived EVs for potential equine therapy.