肺炎克雷伯菌是一种机会性病原体,可引起医院和社区获得性感染。其致病性与多种毒力因子和抗生素耐药性有关。本研究的目的是比较ESBL和非ESBL分离株之间的毒力属性。
■在布什尔省收集了113名肺炎克雷伯菌,其中包括56名ESBL和57名非ESBL生产者,伊朗,从2017年11月到2019年2月。酶谱,对高粘膜粘度和生物膜形成进行了表型研究。此外,RMPA的存在,aerobactin,kfu,allS,mrkD,ybtS,entB,IutA,FIMH,wabG,wcag,通过PCR和测序检测K1和K2基因。
■ESBL生产者和非ESBL生产者之间的酶谱没有统计学上的显着差异。与非ESBL生产者相比,ESBL中的高粘度患病率较低,但ESBL生产者中的生物膜强度较高。在毒力基因中,K1,rmpA,IutA,仅在非ESBLs中观察到空气动力学。此外,所有的马车,K,K2、rmpA、与非高粘膜粘性分离株相比,iutA和aero基因在高粘膜粘性中更高。
■潜在致病性分离株的鉴定在防止其传播以及治疗成功方面起着重要作用。
UNASSIGNED: Klebsiella pneumoniae is an opportunistic pathogen responsible for causing nosocomial and community-acquired infections. Its pathogenicity is associated with a variety of virulence factors and antibiotic resistance. The aim of the present study was to compare virulence attributes between ESBL and non-ESBL producing isolates.
UNASSIGNED: A total of 113 K. pneumoniae including 56 ESBL and 57 non ESBL-producers were collected in Bushehr province, Iran, from November 2017 to February 2019. Enzymatic profile, hypermucoviscosity and biofilm formation were investigated phenotypically. In addition, the presence of rmpA, aerobactin, kfu, allS, mrkD, ybtS, entB, iutA, fimH, wabG, wcaG, K1 and K2 genes were detected by PCR and sequencing.
UNASSIGNED: There was no statistically significant difference in enzymatic profile between ESBL and non-ESBL producers. The prevalence of the hypermocoviscosity was lower among ESBL compared to non-ESBL producers but the intensity of biofilm was higher in the ESBL producers. Among the virulence genes, K1, rmpA, iutA, and aero were observed only in non-ESBLs. Moreover, the carriage of allS, K, K2, rmpA, iutA and aero genes was higher in hypermucoviscous in comparison with non hypermucoviscous isolates.
UNASSIGNED: The identification of potentially pathogenic isolates plays an important role in preventing their spread as well as the success of their treatment.