背景:支原体(M.)猪肺炎与猪的呼吸道疾病有关,是地方性肺炎的主要病原体。猪肺炎支原体相关结果参数的定量可能很困难,贵,耗时,在研究和现场设置中。除了完善的方法,技术工具正在变得可用来监测相关动物和环境相关特征的各个方面,经常是实时的。因此,这项研究旨在评估某些参数,例如使用微芯片(IMT)的动物运动和体温,与已建立的参数相关,以及当前使用的参数是否可以合理化。
结果:猪肺炎支原体感染后,移动百分比显着降低(p<0.05),与阴性对照组(6.9%)相比,猪肺炎支原体感染组的运动百分比(1.9%)较低。另一方面,宏观(MLCL)和微观(MLL)肺部病变,呼吸系统疾病评分(RDS),猪肺炎支原体-DNA载量,和反M接种后28天,猪肺炎支原体感染组的猪肺炎抗体水平显着增加(p<0.05)。在上述参数之间观察到中度(r>0.30)到非常强的相关性(>0.80)(p<0.05),除了IMT。据报道,IMT与直肠温度之间存在显着和中等相关性(r=0.49;p<0.05)。最后,猪肺炎支原体感染对平均日增重和肺部空气百分比没有影响(p>0.05)。
结论:M.猪肺炎的感染显著减少了仔猪的运动,增加了肺部病变,猪肺炎支原体-DNA载量,和反M猪肺炎抗体水平;和,在大多数参数之间观察到良好的相关性,这表明了他们之间的直接关系。因此,我们认为运动的变化可能是猪肺炎支原体感染的可靠指标,并且一组选定的参数-特定于RDS,MLCL,MLL,猪肺炎支原体-DNA载量,反M猪肺炎抗体水平,和运动-在实验条件下评估猪肺炎支原体感染是最佳的。
BACKGROUND: Mycoplasma (M.) hyopneumoniae is associated with respiratory disease in pigs and is the primary agent of enzootic pneumonia. Quantification of M. hyopneumoniae-related outcome parameters can be difficult, expensive, and time-consuming, in both research and field settings. In addition to well-established methods, technological tools are becoming available to monitor various aspects of relevant animal- and environment-related features, often in real-time. Therefore, this study aimed to assess whether certain parameters, such as animal movement and body temperature using microchips (IMT), correlate with established parameters and whether the currently used parameters can be rationalized.
RESULTS: The percentage of movement was significantly reduced by M. hyopneumoniae infection in pigs (p < 0.05), where the M. hyopneumoniae-infected group showed a lower percentage of movement (1.9%) when compared to the negative control group (6.9%). On the other hand, macroscopic (MLCL) and microscopic (MLL) lung lesions, respiratory disease score (RDS), M. hyopneumoniae-DNA load, and anti-M. hyopneumoniae antibody levels increased significantly in the M. hyopneumoniae-infected group 28 days post-inoculation (p < 0.05). Moderate (r > 0.30) to very strong correlations (> 0.80) were observed between the abovementioned parameters (p < 0.05), except for IMT. A significant and moderate correlation was reported between IMT and rectal temperature (r = 0.49; p < 0.05). Last, the average daily weight gain and the percentage of air in the lung were not affected by M. hyopneumoniae infection (p > 0.05).
CONCLUSIONS: M. hyopneumoniae infection significantly reduced the movement of piglets and increased lung lesions, M. hyopneumoniae-DNA load, and anti-M. hyopneumoniae antibody levels; and, good correlations were observed between most parameters, indicating a direct relationship between them. Thus, we suggest that changes in movement might be a reliable indicator of M. hyopneumoniae infection in pigs, and that a selected group of parameters-specifically RDS, MLCL, MLL, M. hyopneumoniae-DNA load, anti-M. hyopneumoniae antibody levels, and movement-are optimal to assess M. hyopneumoniae infection under experimental conditions.