UNASSIGNED:虽然有报道表明中药治疗卵巢癌(OC)有很好的疗效,异鼠李素(ISO)的作用,具有免疫力的黄酮醇糖苷配基,抗炎,心血管和脑血管保护作用,以及抗癌作用,OC尚不清楚。网络药理学被用来在体外和体内探索这一点,并确定相关目标。
UNASSIGNED:通过构建用于基因本体论(GO)和京都基因和基因组百科全书(KEGG)富集分析的药物靶标和疾病基因数据库,筛选了ISO在OC治疗中的常见靶标。通过STRING构建蛋白质-蛋白质相互作用网络。使用在线工具UALCAN进一步分析重叠靶标,以分析基因表达与患者生存和预后之间的相关性。ISO对OC细胞增殖的影响,迁移,并在体内和体外评估侵袭,并通过过表达和敲低ESR1表达来检查雌激素受体1(ESR1)在OC发展中的功能。
未经评估:通过网络药理学分析,筛选出25个与ISO-OC相关的靶基因。OC患者的总体生存率仅与13个高表达重叠基因中ESR1的高表达显着相关。ISO显著抑制增殖,OC细胞在体外迁移和侵袭,在体内抑制肿瘤生长。ESR1过表达显著促进细胞增殖,OC细胞的迁移和侵袭,而ESR1的敲低显示出相反的结果。此外,ESR1的过表达显著逆转了ISO对细胞增殖的抑制作用,OC细胞的迁移和侵袭。
未经证实:我们证实ISO抑制OC细胞增殖,通过靶向ESR1表达的迁移和入侵,为进一步的药理研究提供了理论依据。
UNASSIGNED: Although reports suggest Chinese herbal medicine treatment of ovarian cancer (OC) has a good effect, the role of isorhamnetin (ISO), a flavonol aglycone with immune, anti-inflammatory, cardiovascular and cerebrovascular protective effects, as well as an anticancer effect, in OC remains unclear. Network pharmacology was used to explore this in vitro and in vivo, and to identify relevant targets.
UNASSIGNED: The common targets of ISO in the treatment of OC were screened by constructing drug targets and disease gene databases for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. The protein-protein interaction network was constructed by STRING. Overlapping targets were further analyzed using the online tool UALCAN to analyze the correlation between gene expression and patient survival and prognosis. The effect of ISO on OC cell proliferation, migration, and invasion was assessed in vivo and in vitro, and the function of the estrogen receptor 1 (ESR1) in the development of OC was examined by overexpressing and knocking down ESR1 expression.
UNASSIGNED: Through network pharmacology analysis, 25 target genes related to ISO-OC were screened out. The overall survival rate of OC patients only significantly correlated with high expression of ESR1 among 13 highly expressed overlapping genes. ISO significantly inhibited the proliferation, migration and invasion of OC cells in vitro and inhibited tumor growth in vivo. Overexpression of ESR1 significantly promoted the proliferation, migration and invasion of OC cells, whereas knockdown of ESR1 showed the opposite result. In addition, overexpression of ESR1 significantly reversed the inhibitory effect of ISO on the proliferation, migration and invasion of OC cells.
UNASSIGNED: We confirmed that ISO inhibits OC cell proliferation, migration and invasion by targeting ESR1 expression, which provides a theoretical basis for further pharmacological research.