中药复方制剂成分复杂。作为一种广泛使用的中药注射剂,舒肝宁注射液,其体内过程尚未完全了解。确定血浆蛋白结合率对于药代动力学和药效学研究具有重要意义。在这个实验中,采用平衡透析法结合UPLC-MS/MS技术测定10种组分的血浆蛋白结合率,包括对羟基苯乙酮,咖啡酸,黄芩素,奥木精A,栀子苷,黄芩苷,cynaroside,奥木精A-7-O-β-D-葡糖苷酸,scutellarin,和金丝桃苷,为进一步阐明舒肝宁注射液的体内过程和指导临床用药提供理论依据。结果表明,除了黄芩素和栀子苷,人血浆中其他八种成分的血浆蛋白结合率高于大鼠血浆,并且存在种间差异。在人类血浆中,除了栀子苷,咖啡酸,和黄芩苷,其余7种成分的血浆蛋白结合率均在80%以上,黄芩素和奥木精A超过90%。所有成分都表现出与血浆蛋白的高水平结合,除了栀子苷。
Traditional Chinese medicine(TCM) compound preparations have complex compositions. As a widely used TCM injection, Shuganning Injection, its in vivo processes are not yet fully understood. Determining the plasma protein binding rate is of great significance for pharmacokinetic and pharmacodynamic studies. In this experiment, the equilibrium dialysis method combined with UPLC-MS/MS technology was used to determine the plasma protein binding rates of 10 components, including p-hydroxyacetophenone, caffeic acid, baicalein, oroxylin A, geniposide, baicalin, cynaroside, oroxylin A-7-O-β-D-glucuronide, scutellarin, and hyperoside, in Shuganning Injection in rat and human plasma to provide a theoretical basis for further elucidating the in vivo processes of Shuganning Injection and guiding clinical medication. The results showed that, except for baicalein and geniposide, the plasma protein binding rates of the other eight components were higher in human plasma than in rat plasma, and there were interspecies differences. In human plasma, except for geniposide, caffeic acid, and baicalin, the plasma protein binding rates of the remaining seven components were above 80%, with baicalein and oroxylin A exceeding 90%. All components exhibit a high level of binding to plasma proteins, with the exception of geniposide.