麦角菌核产生有毒的次级代谢产物,麦角生物碱,感染谷类作物和草。麦角生物碱具有两种异构构型:C-8-R-异构体(R-差向异构体),和C-8-S-异构体(S-差向异构体)。Ergot污染的基质,如谷物或草,在分析之前,可以在各种温度下长时间储存,利用,或消费。这项研究评估了在自然污染的小麦中发现的两种构型中六种常见麦角生物碱随时间的浓度(一种,两个,和四个月)在不同的温度(室温,+4°C,和-20°C)使用超高效液相色谱-串联质谱法。数据表明,在室温下,天然污染样品中的总麦角浓度随时间而变化,+4°C,和-20℃。麦角的总浓度增加到第二个月,并在第四个月下降,与温度无关(p<0.05)。总的R-差向异构体浓度似乎比总的S-差向异构体浓度更不稳定。总R和总S-差向异构体浓度的变化可能是由麦角定和麦角定浓度的变化引起的,分别。在实验室或实际农业环境中储存潜在污染的基质时,应考虑时间和温度。应在使用之前对麦角污染的基质进行定量,以确保对麦角浓度的最可靠估计。
Ergot sclerotia produce toxic secondary metabolites, ergot alkaloids, that infect cereal crops and grasses. Ergot alkaloids have two isomeric configurations: the C-8-R-isomer (R-
epimer), and the C-8-S-isomer (S-
epimer). Ergot contaminated matrices, such as cereal grains or grasses, may be stored for extended periods at various temperatures before being analyzed, utilized, or consumed. This study assessed the concentration of six common ergot alkaloids in both configurations found in naturally contaminated wheat over time (one, two, and four months) at different temperatures (room temperature, +4 °C, and -20 °C) using ultra-high-performance liquid chromatography-tandem mass spectrometry. The data indicate that the total ergot concentration within a natural contaminated sample varies over time at room temperature, +4 °C, and -20 °C. The total ergot concentration increased until month two, and decreased at month four, independent of temperature (p < 0.05). The total R-
epimer concentration appeared to be less stable over time than the total S-
epimer concentration. The changes in the total R and total S-
epimer concentrations may have been caused by changes in the ergocristine and ergocristinine concentrations, respectively. Time and temperature should be considered when storing potentially contaminated matrices in a laboratory or practical agriculture situations. Quantification of ergot contaminated matrices should occur prior to their use to ensure the most reliable estimates of the concentration of ergot.