■正确测量血液和尿肌酐水平对于识别和追踪慢性肾病(CKD)是必要的。
■本研究的目的是比较Jaffe和酶方法测量血清和尿液中的肌酐,为了确定它们之间是否有任何统计上的显著差异,以及它们是否反映在肌酐清除率计算和估计的肾小球滤过率(eGFR)上。
■使用Jaffe方法在DimensionRxL生化分析仪上对24至69岁的患者组(N=60;女性=34,男性=26)进行血清和尿液中的肌酐测定,和酶法在综合生化和免疫化学分析仪Architectci8200上,并将获得的水平用于肌酐清除率计算和eGFR。
■这些方法相关性很好,测量血清肌酐(r1=0.990)和测量尿肌酐(r2=0.974)。它们之间没有统计学上的显著差异(p=0.57)。使用不同的方法测量肌酐显示计算的清除率没有统计学上的显着差异(p=0.93),它们显著相关(r=0.9722)。eGFR,使用MDRD和CKD-EPI公式,没有统计学上的显著差异,不管使用的方法。
■除了使用的方法之间的显著相关性之外,使用Jaffe和酶法的结果在测量血清肌酐水平方面没有显着差异,或肌酐清除率和肾小球滤过率。
UNASSIGNED: Correct measuring of blood and urine creatinine level is necessary for identification and tracking of chronic kidney disease (CKD).
UNASSIGNED: The aim of this study is a comparison of Jaffe and enzymatic methods for measuring creatinine in serum and in urine, in order to determine whether there are any statistical significant differences between them, and whether they are reflected on creatinine clearance calculation and estimated glomerular filtration rate (eGFR).
UNASSIGNED: Creatinine in serum and urine was measured for the group of patients (N=60; female=34, male=26) from 24 to 69 years of age by using Jaffe\'s method on Dimension RxL biochemical analyzer, and enzymatic method on integrated biochemical and immunochemical analyzer Architect ci8200, and obtained levels are used for creatinine clearance calculation and eGFR.
UNASSIGNED: The methods correlate well, both in measuring serum creatinine (r 1 = 0.990) and in measuring urine creatinine (r 2 =0.974). There are no statistically significant differences between them (p=0.57). Measuring creatinine using different methods showed no statistically significant differences in the calculated clearances (p=0.93), they significantly correlate (r=0.9722). eGFR, using the MDRD and CKD-EPI formulas, were not statistically significantly different, regardless of the used method.
UNASSIGNED: Apart from significant correlations between the used methods, the results of using the Jaffe and enzymatic methods showed no significant differences at measuring serum creatinine level, or creatinine clearance and glomerular filtration rate.