肠球菌属。已被证明具有胃肠道保护功能;我们最近的结果表明,膜囊泡(MVs)在屎肠球菌的胃保护中起重要作用(E.faecium)。特定功能由MV的分子组成决定。为了解决屎肠球菌MVs(EfmMVs)中的生物货物成分,从屎肠球菌培养物中分离MV。转录组学,无标记定量蛋白质组学,和非靶向代谢组学进行,以获得有关核糖核酸(RNA)的复杂性的信息,蛋白质,和它们携带的代谢物生物货物,分别。RNA测序鉴定了总共2122个转录物。排名前20位的成绩单占总数的27.63%,which,包括酶,参与糖酵解,核糖体蛋白,DNA定向RNA聚合酶,蛋白质合成相关酶,与蛋白质翻译后加工和转运相关的分子,和肽聚糖裂解酶。无标记定量蛋白质组学分析鉴定出总共711种蛋白质。前20种蛋白质占所有鉴定蛋白质的48.02%,包括核糖体蛋白,酶参与糖酵解,DNA定向RNA聚合酶,蛋白质合成相关酶,肽聚糖裂解酶,和自溶素。非靶向代谢组学分析鉴定出总共519种代谢物。前20种代谢物占所有已鉴定代谢物的79.55%,其中包括氨基酸,基材,或氨基酸代谢的产物,天然有机酸,有机酸代谢中的产物,酮化合物,还有另外两种化合物.基因本体论和京都百科全书的基因和基因组途径分析表明,鉴定的生物货物成分富含代谢,遗传,和环境信息处理。总的来说,我们希望当前对这种EfmMV的多种“组学”分析的探索将为未来关于屎肠球菌应用的研究提供有用的信息和进一步的基础。
Enterococcus spp. have been shown to have gastrointestinal tract protective functions; our recent results suggest that membrane vesicles (MVs) play an important role in the gastric protection of Enterococcus faecium (E. faecium). The specific function is determined by molecular compositions of MVs. To resolve biocargo components in E. faecium MVs (EfmMVs), MVs were isolated from E. faecium culture. Transcriptomics, label-free quantitative proteomics, and untargeted metabolomics were performed to obtain information about the complexity of ribonucleic acids (RNAs), proteins, and metabolites biocargo they carry, respectively. RNA-sequencing identified a total of 2122 transcripts. The top 20 transcripts accounted for 27.63% of total counts, which, including enzymes, participate in glycolysis, ribosomal proteins, DNA-directed RNA polymerases, protein-synthesizing relative enzymes, molecules associated with protein post-translational processing and transport, and peptidoglycan lyases. Label-free quantitative proteomics analysis identified a total of 711 proteins. The top 20 proteins accounted for 48.02% of all identified proteins, which including ribosomal proteins, enzymes participate in glycolysis, DNA-directed RNA polymerases, protein-synthesizing relative enzymes, peptidoglycan lyases, and autolysin. Untargeted metabolomics analysis identified a total of 519 metabolites. The top 20 metabolites accounted for 79.55% of all identified metabolites, which included amino acids, substrates, or products in the metabolism of amino acids, natural organic acids, products in the metabolism of organic acids, ketone compounds, and two other compounds. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses indicated that the identified biocargo components enriched in metabolism, genetic, and environmental information processing. Overall, we hope that the current exploration of multiple \"-omics\" analyses of this EfmMVs will provide useful information and further groundwork for future studies on E. faecium application.