目的:这项研究的目的是研究再矿化剂,如氟化物清漆和P11-4,单独和与Er:YAG激光联合使用的效果,人工牙釉质病变的体外硬组织修复。
方法:在30颗拔除的智齿的颊侧和舌侧创建了总共60个4×5mm大小的釉质表面。将再矿化剂应用于如下分组的样本:第1组,对照;第2组,氟化物清漆(FV);第3组,P11-4;第4组,激光;第5组,激光+FV;和第6组,激光+P11-4。用DiagnoDent测定荧光水平。搪瓷矿物密度,面积和体积,用显微计算机断层扫描(µCT)确定龋齿病变面积和体积,使用扫描电子显微镜(SEM)评估表面特征,并且使用能量色散X射线光谱法(EDS)进行元素分析。
结果:对于仅用自组装肽P11-4处理的标本,治疗前和治疗后的龋损面积(mm2)值分别为38.19和21.62,龋损体积(mm3)值分别为6.27和2.99。在自组装肽P11-4和激光的组合使用中,治疗前后的龋损面积(mm2)值分别为38.39和16.91,龋损体积(mm3)值分别为11.15和3.64.在P11-4单独和与激光结合的应用中,诊断值在统计学上显着下降,搪瓷体积(mm3)的增加,釉质面积(mm2)和矿物质密度(g/cm3)值以及通过µCT获得的龋齿病变体积(mm3)和面积(mm2)的减少,以及通过SEM/EDS分析获得的%Ca和%F值的增加(p<0.05)。发现用P11-4处理的样品具有比用FV处理的样品高得多的Ca/P比的升高(p<0.05)。当P11-4应用与激光照射结合时,钙含量显着增加更多(p<0.05)。
结论:自组装肽P11-4和激光的联合使用加速了再矿化过程并增加了再矿化能力。
结论:FV和P11-4,单独或与激光联合使用,可以成功地用作初始釉质龋齿的再矿化剂。
OBJECTIVE: The aim of this study was to investigate the effect of remineralization agents such as fluoride varnish and P11-4, alone and in combination with Er: YAG laser, on in-vitro hard tissue repair in artificial enamel lesions.
METHODS: A total of sixty enamel surfaces of 4 × 5 mm in size were created on both the buccal and lingual sides of thirty extracted wisdom teeth. Remineralization agents were applied to the specimens that were grouped as follows: Group 1, control; Group 2, fluoride varnish (FV); Group 3, P11-4; Group 4, laser; Group 5, laser + FV; and Group 6, laser + P11-4. The fluorescence level was determined with DiagnoDent. The enamel mineral density, area and volume, and caries lesion area and volume were determined with micro-computed tomography (µCT), surface features were evaluated using scanning electron microscopy (SEM), and elemental analysis was performed using energy dispersive x-ray spectroscopy (EDS) .
RESULTS: For specimens treated only with self-assembling peptide P11-4, the caries lesion area (mm2) values were 38.19 and 21.62, and the caries lesion volume (mm3) values were 6.27 and 2.99, respectively for pre- and post-treatment. In combination usage of self-assembling peptide P11-4 and laser, the caries lesion area (mm2) values were 38.39 and 16.91, and the caries lesion volume (mm3) values were 11.15 and 3.64, respectively for pre- and post-treatment. In the application of the P11-4 alone and in combination with laser, there was a statistically significant decrease in DiagnoDent values, an increase in enamel volume(mm3),enamel area(mm2) and mineral density(g/cm3) values and a decrease in caries lesion volume(mm3) and area(mm2) obtained by µCT, and an increase in %Ca and %F values obtained by SEM/EDS analysis (p < 0.05). It was discovered that the samples treated with P11-4 had a considerably higher rise in the Ca/P ratio than the samples treated with FV (p < 0.05). The calcium content increased significantly more when P11-4 application was combined with laser irradiation (p < 0.05).
CONCLUSIONS: The combined use of self-assembling peptide P11-4 and laser accelerated the remineralization process and increased the remineralization capacity.
CONCLUSIONS: FV and P11-4, alone or in combination with laser, can be successfully used as remineralization agents in initial enamel caries.