■病毒通常会引起疾病,但是一些病毒作为宿主或宿主微生物组的常驻调节者可能是有益的。植物相关病毒可以通过增加胁迫耐受性或调节内生群落来帮助植物生存。这项研究的目的是表征香蕉和车前草中的内生病毒群落(Musaspp。)基因型,包括栽培和野生物种,评估病毒库并检测新病毒。
■DNA病毒群落的特征是对7种不同Musa基因型的叶子和根或球茎的富集内圈提取物进行shot弹枪测序(M.balbisiana,泰国黑,M.textilis,Sikkimensis,矮人卡文迪许,威廉姆斯混合动力车和FHIA-25混合动力)。
■结果显示丰富的病毒样重叠群长达108,191bp,叶片中的相对丰度高于根中的相对丰度。分析预测了51个家族的733个噬菌体物种,植物之间的噬菌体群落几乎没有重叠。根和二倍体野生宿主的噬菌体多样性较高。阿克曼病毒科和根瘤菌噬菌体通常是最丰富的类群。发现了与内生肿瘤引起根瘤菌的噬菌体相关的根瘤菌RR1样噬菌体,带有holin基因和部分志贺样毒素基因,引起人们对其调节内生根瘤菌科的潜力的兴趣。克雷伯菌噬菌体可能对枯萎病有保护作用,和其他噬菌体被预测具有调节欧文氏菌的潜力,拟杆菌,和拉斯特氏菌相关的疾病。尽管功能上注释了丰富的含噬菌体的重叠群,揭示了1038个预测的病毒蛋白结构域,基因库显示出与数据库序列的高度分歧,提示这些香蕉品种中的新型噬菌体。植物DNA病毒包括56种Badnavirus和26种其他非Musa植物病毒,其分布表明这些样品中存在常驻和瞬时植物DNA病毒的混合物。
■一起,来自共享环境的这些植物中不同的病毒群落表明宿主驱动着这些病毒群落的组成。这项研究是了解这种全球重要粮食作物中内生病毒的第一步,目前受到真菌的威胁,细菌,和病毒性疾病。
UNASSIGNED: Viruses generally cause disease, but some viruses may be beneficial as resident regulators of their hosts or host microbiomes. Plant-associated viruses can help plants survive by increasing stress tolerance or regulating endophytic communities. The goal of this study was to characterize endophytic virus communities in banana and plantain (Musa spp.) genotypes, including cultivated and wild species, to assess virome repertoires and detect novel viruses.
UNASSIGNED: DNA viral communities were characterized by shotgun sequencing of an enriched endosphere extract from leaves and roots or corm of 7 distinct Musa genotypes (M. balbisiana, Thai Black, M. textilis, M. sikkimensis, Dwarf Cavendish, Williams Hybrid, and FHIA-25 Hybrid).
UNASSIGNED: Results showed abundant virus-like contigs up to 108,191 bp long with higher relative abundance in leaves than roots. Analyses predicted 733 phage species in 51 families, with little overlap in phage communities among plants. Phage diversity was higher in roots and in diploid wild hosts. Ackermanniviridae and Rhizobium phage were generally the most abundant taxa. A Rhizobium RR1-like phage related to a phage of an endophytic tumor-causing rhizobium was found, bearing a holin gene and a partial Shiga-like toxin gene, raising interest in its potential to regulate endophytic Rhizobiaceae. Klebsiella phages were of interest for possible protection against Fusarium wilt, and other phages were predicted with potential to regulate Erwinia, Pectobacterium, and Ralstonia-associated diseases. Although abundant phage-containing contigs were functionally annotated, revealing 1,038 predicted viral protein domains, gene repertoires showed high divergence from database sequences, suggesting novel phages in these banana cultivars. Plant DNA viruses included 56 species of Badnavirus and 26 additional non-Musa plant viruses with distributions that suggested a mixture of resident and transient plant DNA viruses in these samples.
UNASSIGNED: Together, the disparate viral communities in these plants from a shared environment suggest hosts drive the composition of these virus communities. This study forms a first step in understanding the endophytic virome in this globally important food crop, which is currently threatened by fungal, bacterial, and viral diseases.