■成纤维细胞生长因子(FGFs)在胚胎植入中起关键作用,并支持子宫内膜滋养细胞的相互作用。
■该研究的目的是评估FGF-1(rs34011)基因种类及其血清浓度与反复植入失败(RIF)之间的关联。
■研究的设计是横断面研究。
400名有RIF病史的不育妇女和400名接受首次体外受精-胚胎移植并成功分娩的健康妇女(对照)被纳入研究。从外周血白细胞中提取基因组DNA,并通过四引物扩增难治性突变系统-聚合酶链反应进行基因分型。采用酶联免疫吸附法检测血清FGF-1浓度。
使用ANOVA检验分析各组均值之间的差异。
■在RIF组中,GG的基因型频率,GA和AA分别为59%,33.5%和7.5%,分别,而对照组为72.5%,24%和3.5%,分别。RIF组G和A等位基因频率分别为75.75%和24.25%,而在对照组中分别为84.5%和15.5%,分别为(P<0.0001)。我们还显示RIF和对照组的血清FGF-1浓度为17±3.55和23.62±4.91pg/mL,分别为(P=0.008)。我们还表明,AA基因型与RIF中血清FGF-1浓度降低显着相关(AA,GA和GG血清水平分别为9.55±2.65,14±3.35和22.55±7.26pg/mL,对照组分别为12.22±2.27、18.44±5.98和26.66±8.29pg/mL,分别)。
■目前的研究表明,FGF-1(rs34011)启动子多态性及其血清浓度与RIF之间存在显着关联。该研究还表明AA基因型与较低的FGF-1血清水平有关,并且可能是RIF的危险因素。
UNASSIGNED: Fibroblast growth factors (FGFs) play a key role in embryo implantation and support endometrial trophoblastic interaction.
UNASSIGNED: The aim of the study was to evaluate the association between FGF-1 (rs34011) gene variety and its serum concentration with repeated implantation failure (RIF).
UNASSIGNED: The design of the study was a cross-sectional study.
UNASSIGNED: Four hundred infertile women with a history of RIF and 400 healthy women undergoing the first in vitro fertilisation-embryo transfer attempt with successful delivery (controls) were enrolled in the study. Genomic DNA was extracted from peripheral blood leucocytes and genotyped by Tetra-Primer Amplification Refractory Mutation System-Polymerase Chain Reaction. Serum FGF-1 concentration was evaluated with enzyme-linked immunosorbent assay.
UNASSIGNED: The ANOVA test was used to analyse the difference between the means of the groups.
UNASSIGNED: In RIF group, the genotype frequencies of the GG, GA and AA were 59%, 33.5% and 7.5%, respectively, whereas in controls were 72.5%, 24% and 3.5%, respectively. The G and A allele frequencies in the RIF group were 75.75% and 24.25%, while in controls were 84.5% and 15.5%, respectively (P < 0.0001). We have also shown that serum FGF-1 concentration in RIF and control groups was 17 ± 3.55 and 23.62 ± 4.91 pg/mL, respectively (P = 0.008). We have also shown that AA genotype is significantly associated with decreased serum FGF-1 concentration in RIF (AA, GA and GG serum levels were 9.55 ± 2.65, 14 ± 3.35 and 22.55 ± 7.26 pg/mL, and in controls were 12.22 ± 2.27, 18.44 ± 5.98 and 26.66 ± 8.29 pg/mL, respectively).
UNASSIGNED: The current study suggests that a significant association between FGF-1 (rs34011) promoter polymorphism and its serum concentration with RIF. The study also suggests that AA genotype is linked to lower FGF-1 serum levels and may play a risk factor for RIF.