■土壤真菌群落在维持高山森林土壤生态系统的生态功能中起着至关重要的作用。然而,目前尚不清楚高山森林不同土壤层中真菌群落的分布格局将如何沿海拔梯度变化。
■因此,IlluminaMiSeq测序技术用于研究Sygera山脉沿海拔梯度(3500m至4300m)的三个土壤层(0-10、10-20和20-30cm)中的真菌群落,位于八一区,林芝市,西藏。
■结果表明:1)土壤深度对真菌多样性的影响大于海拔,表明随着土壤深度的增加,真菌多样性显着降低,但在所有土壤层中,海拔变化均无显着差异。在0-10厘米的土壤层内,担子菌和子囊菌共同主导着微生物群落。然而,随着土壤深度增加到10-20和20-30厘米的土壤层,担子菌主要占主导地位。2)确定过程在0-10cm真菌群落的组装机制中占主导地位,并且随着海拔的增加而保持不变。相比之下,随着海拔的增加,10-20和20-30厘米真菌群落的组装机制从确定性过程转变为随机过程。3)0-10厘米真菌群落的网络复杂度随着海拔的升高而逐渐增加,而10-20和20-30cm真菌群落表现出减少的趋势。与0-10厘米的土层相比,在10-20和20-30厘米的土壤层中,真菌生物标志物的相对丰度发生了更多的变化,表明这些深度的真菌群落对气候变化更敏感。在推动这些改变的关键因素中,土壤温度和湿度土壤水分含量在塑造真菌群落的组装机制和网络复杂性方面至关重要。这项研究有助于理解高寒生态系统中土壤真菌群落格局和沿海拔梯度的驱动因素,并为预测高寒森林中土壤微生物生态系统的功能响应提供了重要的科学依据。
UNASSIGNED: Soil fungal communities play a crucial role in maintaining the ecological functions of alpine forest soil ecosystems. However, it is currently unclear how the distribution patterns of fungal communities in different soil layers of alpine forests will change along the elevational gradients.
UNASSIGNED: Therefore, Illumina MiSeq sequencing technology was employed to investigate fungal communities in three soil layers (0-10, 10-20, and 20-30 cm) along an elevational gradient (3500 m to 4300 m) at Sygera Mountains, located in Bayi District, Nyingchi City, Tibet.
UNASSIGNED: The results indicated that: 1) Soil depth had a greater impact on fungal diversity than
elevation, demonstrating a significant reduction in fungal diversity with increased soil depth but showing no significant difference with
elevation changes in all soil layers. Within the 0-10 cm soil layer, both Basidiomycota and Ascomycota co-dominate the microbial community. However, as the soil depth increases to 10-20 and 20-30 cm soil layers, the Basidiomycota predominantly dominates. 2) Deterministic processes were dominant in the assembly mechanism of the 0-10 cm fungal community and remained unchanged with increasing
elevation. By contrast, the assembly mechanisms of the 10-20 and 20-30 cm fungal communities shifted from deterministic to stochastic processes as
elevation increased. 3) The network complexity of the 0-10 cm fungal community gradually increased with
elevation, while that of the 10-20 and 20-30 cm fungal communities exhibited a decreasing trend. Compared to the 0-10 cm soil layer, more changes in the relative abundance of fungal biomarkers occurred in the 10-20 and 20-30 cm soil layers, indicating that the fungal communities at these depths are more sensitive to climate changes. Among the key factors driving these alterations, soil temperature and moisture soil water content stood out as pivotal in shaping the assembly mechanisms and network complexity of fungal communities. This study contributes to the understanding of soil fungal community patterns and drivers along elevational gradients in alpine ecosystems and provides important scientific evidence for predicting the functional responses of soil microbial ecosystems in alpine forests.