心脏组织工程(CTE)是医疗保健系统中修复受损心脏组织的一种有前途的方法。用适当的化学方法开发可生物降解的支架,电气,机械,和生物学特性是CTE成功的未满足需求。静电纺丝是一种多功能技术,已在CTE中显示出潜在的应用。在这里,四种不同类型的多功能脚手架,包括合成基聚(癸二酸甘油酯)-聚氨酯(PGU),PGU-大豆脚手架,以及一系列三层支架,其中包含两个PGU-Soy外层和一个明胶(G)中间(内部)层,作为天然且可生物降解的大分子,没有辛伐他汀(S)和辛伐他汀(GS),一种抗炎药,使用静电纺丝技术在三明治状结构中制造。这种方法提供了合成和天然聚合物两者的优点的组合,以增强生物活性以及细胞与细胞和细胞与基质的相互连通。在掺入大豆油(Soy)和G后进行体外药物释放分析。引入大豆作为半导体材料以改善纳米纤维支架的电导率。物理化学性质,接触角,还评估了静电纺丝支架的生物降解性。此外,通过活化部分凝血活酶时间(APTT)研究纳米纤维支架的血液相容性,凝血酶原时间(PT),和溶血试验。结果表明,所有支架均表现出无缺陷的形态,平均纤维直径为361±109至417±167nm。观察到血液凝固延迟,证明了纳米纤维支架的抗凝血性质。此外,大鼠心肌细胞系(H9C2)在支架上培养7天,并监测细胞形态和排列。数据表明适当的细胞相容性。值得注意的是,在PGU-大豆/GS纳米纤维支架中,与其他组相比,显示出较高的生存率。我们的发现表明,辛伐他汀负载的聚合物系统对心肌细胞的附着和生长具有积极作用,可以用作CTE领域的药物释放载体。
Cardiac tissue engineering (CTE) is a promising way for the restoration of injured cardiac tissue in the healthcare system. The development of biodegradable scaffolds with appropriate chemical, electrical, mechanical, and biological properties is an unmet need for the success of CTE. Electrospinning is a versatile technique that has shown potential applications in CTE. Herein, four different types of multifunctional scaffolds, including synthetic-based poly (glycerol sebacate)-polyurethane (PGU), PGU-Soy scaffold, and a series of trilayer scaffolds containing two outer layers of PGU-Soy and a middle (inner) layer of gelatin (G) as a natural and biodegradable macromolecule without simvastatin (S) and with simvastatin (GS), an anti-inflammatory agent, were fabricated in the sandwich-like structure using electrospinning technique. This approach offers a combination of the advantages of both synthetic and natural polymers to enhance the bioactivity and the cell-to-cell and cell-to-matrix intercommunication. An in vitro drug release analysis was performed after the incorporation of soybean oil (Soy) and G. Soy is used as a semiconducting material was introduced to improve the electrical conductivity of nanofibrous scaffolds. The physicochemical properties, contact angle, and biodegradability of the electrospun scaffolds were also assessed. Moreover, the blood compatibility of nanofibrous scaffolds was studied through activated partial thromboplastin time (APTT), prothrombin time (PT), and hemolytic assay. The results showed that all scaffolds exhibited defect-free morphologies with mean fiber diameters in the range of 361 ± 109 to 417 ± 167 nm. A delay in blood clotting was observed, demonstrating the anticoagulant nature of nanofibrous scaffolds. Furthermore, rat cardiomyoblast cell lines (H9C2) were cultured on scaffolds for 7 days, and the morphology and cell arrangement were monitored. Data indicated an appropriate cytocompatibility. Of note, in the PGU-Soy/GS nanofibrous scaffold, a high survival rate was indicated compared to other groups. Our findings exhibited that the simvastatin-loaded polymeric system had positive effects on cardiomyoblasts attachment and growth and could be utilized as a drug release carrier in the field of CTE.