胶原蛋白,一种天然存在的纤维蛋白质,是组织工程和再生医学生物材料的潜在资源,因为它在结构上具有生物相容性,免疫原性低,是可生物降解的,而且是仿生的.许多研究已经在文献中记录了胶原蛋白纳米纤维如何表现出有限的细胞粘附,粘度差,没有内部纤维结构。生物医学行业正在使用聚甘油癸二酸酯预聚物(PGSp),一种生物可降解和生物相容性聚酯,具有高粘附性和非常粘稠的外观,更多的时候。这里,开发了用于皮肤组织应用的独特的电纺胶原蛋白/PGSp/ZnO/NPs共混纳米纤维,并描述了不同的PGSp百分比。此外,当胶原蛋白的三元混合物,PGSp,使用了氧化锌纳米颗粒(ZnONPs),提高了支架的抗菌性能。通过将PGSp浓度提高到30%w/w来生产无珠电纺纳米纤维。SEM,EDS,拉伸,MTT,FTIR,SDS-page,肿胀试验,接触角,抗菌,生物降解,XRD,和细胞附着程序用于表征交联的纳米纤维。胶原蛋白/PGSp30%/ZnONPs1%重量比的三元共混纳米纤维具有较高的应力/应变强度(0.25mm/mm),孔隙度(563),细胞存活,和退化时间。此外,在糖尿病大鼠中应用伤口愈合后,与其他组相比,胶原蛋白/PGSp为30%/可以显着改善伤口愈合。
Collagen, a naturally occurring fibrous protein, is a potential resource of biological materials for tissue engineering and regenerative medicine because it is structurally biocompatible, has low immunogenicity, is biodegradable, and is biomimetic. Numerous studies have documented in the literature how Collagen nanofibers exhibit limited cell adhesion, poor viscosity, and no interior fibril structure. The biomedical industry is using Poly Glycerol Sebacate prepolymer(PGSp), a biodegradable and biocompatible polyester with high adhesion and very viscous appearance, more often. Here, unique
electrospun Collagen/PGSp/ZnO/NPs blend nanofibers for skin tissue application were developed and described with varied PGSp percent. Additionally, when ternary blends of Collagen, PGSp, and Zink Oxide Nanoparticles (ZnO NPs) are used, the antibacterial properties of the scaffolds are improved. The bead-free
electrospun nanofibers were produced by raising the PGSp concentration to 30%w/w. SEM, EDS, tensile, MTT, FTIR, SDS-page, swelling test, contact-angle, antimicrobial, biodegradation, XRD, and cell attachment procedures were used to characterize the crosslinked nanofibers. The ternary blend nanofibers with a weight ratio of Collagen/PGSp 30%/ZnONPs 1% had higher stress/strain strength (0.25 mm/mm), porosity (563), cell survival, and degradation time. Moreover, after applying for wound healing in diabetic rats, Collagen/PGSp 30%/could be show improving wound healing significantly compared to other groups.