以稻瘟病中的tenuazonic酸(TeA)合成酶1(TAS1)为参考,同源物AaTAS1首先通过从头测序锚定在链格孢菌中。随后,AaMFS1作为邻近上游区域的主要促进因子超家族(MFS)蛋白编码基因,被关注着。正如假设的那样,AATAS1是TeA生物合成所必需的,而AaMFS1是用于TeA跨膜运输的外排泵。相对而言,与野生型菌株相比,ΔAaTAS1和ΔAaMFS1的TeA产量显着下降。具体来说,AaTAS1的A域在体外催化TeA生物合成的启动。同时,ΔAaTAS1的致病性也显着降低。转录组分析证实了产生TeA的表型和相关基因表达之间的上述一致性。此外,AaTAS1和AaMFS1蛋白存在于细胞质中,质膜,和细胞内膜系统,分别,通过荧光定位。即,AaTAS1负责TeA的生物合成,AaMFS1负责TeA的外排转运。当然,AaTAS1通过合成TeA水平间接调控AaMFS1的表达。总的来说,有关新型AaTAS1和AaMFS1基因的数据主要有助于霉菌毒素生物合成和植物病原体对农业食品的致病性的理论进展。[公式:见正文]版权所有©2022作者(S)。这是在CCBY-NC-ND4.0国际许可证下分发的开放访问文章。
Taking tenuazonic acid (TeA) synthetase 1 (TAS1) in Pyricularia oryzae as a reference, the homolog AaTAS1 was first anchored in Alternaria alternata via de novo sequencing. Subsequently, AaMFS1, as a major facilitator superfamily (MFS) protein-encoding gene in the adjacent upstream region, was followed with interest. As hypothesized, AaTAS1 is required for TeA biosynthesis, while AaMFS1 is an efflux pump for the transmembrane transport of TeA. Comparatively, the TeA yield of ΔAaTAS1 and ΔAaMFS1 dropped significantly compared with that of the wild-type strain. Specifically, the A domain of AaTAS1 catalyzed the start of TeA biosynthesis in vitro. Simultaneously, the pathogenicity of ΔAaTAS1 was also significantly decreased. Transcriptome analysis confirmed the abovementioned consistency between the TeA-producing phenotypes and related gene expression. Moreover, the proteins AaTAS1 and AaMFS1 were found present in the cytoplasm, plasma membrane, and intracellular membrane system, respectively, by fluorescence localization. Namely, AaTAS1 was responsible for the biosynthesis of TeA, and AaMFS1 was responsible for the efflux transport of TeA. Certainly, AaTAS1 indirectly regulated the expression of AaMFS1 through the level of synthetic TeA. Overall, data on the novel AaTAS1 and AaMFS1 genes mainly contribute to theoretical advances in mycotoxin biosynthesis and the pathogenicity of phytopathogens to agricultural foods.[Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.