目的:本研究评估外周血来源的细胞外囊泡(EV)中microRNA-200(miR-200)表达在早期非小细胞肺癌(NSCLC)中的诊断价值。
方法:本研究回顾性分析了100名健康志愿者(对照组),168例早期NSCLC患者(NSCLC组),和128例良性肺结节患者(良性组)。获得参与者的基础和临床数据,包括年龄,性别,吸烟史,糖类抗原242(CA242),癌胚抗原(CEA),糖类抗原199(CA199),1s内用力呼气量,最大自愿通气,强制肺活量,白细胞介素-6(IL-6),肿瘤坏死因子-α(TNF-α),和miR-200表达。外周血来源的电动汽车miR-200表达与CA242、CEA、分析了CA199,评估外周血来源的EVmiR-200对早期NSCLC的诊断价值。还确定了早期NSCLC发展的危险因素。
结果:年龄,有吸烟史的患者百分比,CA242,CEA,CA199、IL-6和TNF-α水平,外周血来源的EV中miR-200的表达在NSCLC组中显著高于良性组和对照组。外周血来源的EV中miR-200高表达的肺部疾病患者占吸烟史和混合病变患者的比例较高,CA242,CEA较高,在外周血来源的电动汽车中,CA199和TNF-α水平高于miR-200低表达的水平。在肺部疾病中,miR-200在外周血来源的EV中的表达与CA242、CEA、CA199外周血来源的EVmiR-200联合CA242、CEA和CA199的诊断价值(曲线下面积=0.942)高于单一检测,随着更高的特异性,外周血来源的EVmiR-200的高表达是早期NSCLC的独立危险因素。
结论:肺部疾病患者外周血来源的EV-200表达与CA242、CEA、和CA199,外周血来源的EVmiR-200的高表达是早期NSCLC的独立危险因素,对早期NSCLC具有较高的临床诊断价值。
OBJECTIVE: This study assessed the diagnostic value of microRNA-200 (miR-200) expression in peripheral blood-derived extracellular vesicles (EVs) in early-stage non-small cell lung cancer (NSCLC).
METHODS: This study retrospectively analyzed 100 healthy volunteers (the control group) receiving physical examinations, 168 early-stage NSCLC patients (the NSCLC group), and 128 patients with benign lung nodules (the benign group). The basic and clinical data of participants were obtained, including age, sex, smoking history, carbohydrate antigen 242 (CA242), carcinoembryonic antigen (CEA), carbohydrate antigen 199 (CA199), forced expiratory volume in 1 s, maximal voluntary ventilation, forced vital capacity, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and miR-200 expression. The correlation of miR-200 expression in peripheral blood-derived EVs with CA242, CEA, and CA199 was analyzed, and the diagnostic value of peripheral blood-derived EV miR-200 for early-stage NSCLC was assessed. The risk factors of early-stage NSCLC development were also determined.
RESULTS: Age, the percentage of patients with smoking history, CA242, CEA, CA199, IL-6, and TNF-α levels, and miR-200 expression in peripheral blood-derived EVs were significantly higher in the NSCLC group than in the benign and control groups. Lung disease patients with high miR-200 expression in peripheral blood-derived EVs comprised a higher percentage of patients with smoking history and mixed lesions and had higher CA242, CEA, CA199, and TNF-α levels than those with low miR-200 expression in peripheral blood-derived EVs. In lung diseases, miR-200 expression in peripheral blood-derived EVs was significantly and positively correlated with CA242, CEA, and CA199. Peripheral blood-derived EV miR-200 combined with CA242, CEA and CA199 had higher diagnostic value (area under the curve = 0.942) than single detection, along with higher specificity, and high expression of peripheral blood-derived EV miR-200 was an independent risk factor for early-stage NSCLC.
CONCLUSIONS: Peripheral blood-derived EV miR-200 expression in patients with lung diseases is closely correlated with CA242, CEA, and CA199, and high expression of peripheral blood-derived EV miR-200 is an independent risk factor for early-stage NSCLC and is of high clinical diagnostic value for early-stage NSCLC.