随着年龄的增长,骨骼肌质量和功能趋于下降。胰岛素样生长因子1(IGF-1)在促进骨骼肌生长中起关键作用。运动通过激活IGF-1信号改善骨骼肌质量和功能。这项研究的目的是调查不同类型的运动是否可以促进肌肉肥大,运动和代谢能力,并激活早期衰老小鼠的IGF-1信号。12月龄雄性C57/BL6小鼠随机分为5组:对照组(CON),有氧运动组(AE),抵抗运动组(RE),全身振动组(WBV)和电刺激组(ES)。肌肉重量,肌纤维大小,IGF-1信号的水平,氧化应激,蛋白质的合成和降解,并检测腓肠肌细胞凋亡。C2C12细胞用于探索机制。在这项研究中,我们证实了四种运动方式增加了骨骼肌质量,锻炼能力,代谢和蛋白质合成的指标,并通过激活IGF-1通路抑制氧化应激和细胞凋亡。最有效的干预措施是RE。我们发现WBV比AE更好地促进肌肉肥大。此外,体外实验,进一步证实了IGF-1/IGF-1R-PI3K/Akt信号传导对于维持骨骼肌质量的重要性.AE,RE,WBV和ES增加骨骼肌质量,锻炼能力,蛋白质合成和代谢酶活性,通过激活IGF-1信号抑制早期衰老小鼠的蛋白质降解和凋亡。其中,WBV已被证明是显著有效的,并且在促进肌肉肥大方面具有与常规运动类似的效果。
Skeletal muscle mass and function tend to decline with increasing age. Insulin-like growth factor 1 (IGF-1) plays a key role in promoting skeletal muscle growth. Exercise improves skeletal muscle mass and function via activating the IGF-1 signaling. The aim of this study was to investigate whether different types of exercise could promote muscle hypertrophy, exercise and metabolic capacities, and activate IGF-1 signaling in early aging mice. 12-month-old male C57/BL6 mice were randomly divided into five groups: control group (CON), aerobic exercise group (AE), resistance exercise group (RE), whole-body vibration group (WBV) and electrical stimulation group (ES). Muscle weight, myofiber size, levels of IGF-1 signaling, oxidative stress, protein synthesis and degradation, and apoptosis in gastrocnemius muscle were detected. C2C12 cells were used to explore the mechanism. In this study, we confirmed that four modes of exercise increased skeletal muscle mass, exercise capacity, indicators of metabolism and protein synthesis, and inhibited oxidative stress and apoptosis via activating the IGF-1 pathway. The most effective intervention was RE. We found that WBV promoted muscle hypertrophy better than AE. Furthermore, in vitro experiment, the importance of IGF-1 / IGF-1R-PI3K / Akt signaling for maintaining skeletal muscle mass was further confirmed. AE, RE, WBV and ES increase skeletal muscle mass, exercise capacities, protein synthesis and metabolic enzyme activities, inhibit protein degradation and apoptosis in mice undergoing early aging via activating IGF-1 signaling. Among them, WBV has been shown to be significantly effective and has a similar effect of conventional exercise in promoting muscle hypertrophy.