黄曲霉毒素是最臭名昭著的霉菌毒素之一,其中黄曲霉毒素B1(AFB1)是最有害和最普遍的。微生物在AFB1生物转化的环境中起着至关重要的作用。在这项研究中,一个细菌财团,获得了能够降解和解毒AFB1的HS-1。这里,我们将多组学和基于培养的技术结合起来,阐明了由HS-1财团进行的AFB1生物转化.共现网络分析显示,HS-1财团中负责AFB1生物转化的关键类群主要属于门变形杆菌和放线菌。此外,宏基因组分析表明,不同的微生物,主要属于门变形杆菌和放线菌,携带AFB1生物转化的初始步骤中涉及的关键功能酶。代谢组学分析表明,副球菌相关细菌在HS-1聚生体中最活跃。一种新型细菌,Paracocussp.从财团HS-1中分离出的XF-30菌株含有一种能够有效降解AFB1的新型染料脱色过氧化物酶(DyP)酶。生物信息学分类分析显示,DyP,涉及AFB1的初始生物转化,广泛分布在各种环境的宏基因组中,主要在分类学上与变形杆菌和放线菌有关。对HS-1财团中AFB1生物转化的深入研究将有助于我们更敏感和有效地探索这些关键生物资源。
Aflatoxin is one of the most notorious mycotoxins, of which aflatoxin B1 (AFB1) is the most harmful and prevalent. Microbes play a crucial role in the environment for the biotransformation of AFB1. In this study, a bacterial consortium, HS-1, capable of degrading and detoxifying AFB1 was obtained. Here, we combined multi-omics and cultivation-based techniques to elucidate AFB1 biotransformation by consortium HS-1. Co-occurrence network analysis revealed that the key taxa responsible for AFB1 biotransformation in consortium HS-1 mainly belonged to the phyla Proteobacteria and Actinobacteria. Moreover, metagenomic analysis showed that diverse microorganisms, mainly belonging to the phyla Proteobacteria and Actinobacteria, carry key functional enzymes involved in the initial step of AFB1 biotransformation. Metatranscriptomic analysis indicated that Paracoccus-related bacteria were the most active in consortium HS-1. A novel bacterium, Paracoccus sp. strain XF-30, isolated from consortium HS-1, contains a novel dye-decolorization peroxidase (DyP) enzyme capable of effectively degrading AFB1. Taxonomic profiling by bioinformatics revealed that DyP, which is involved in the initial biotransformation of AFB1, is widely distributed in metagenomes from various environments, primarily taxonomically affiliated with Proteobacteria and Actinobacteria. The in-depth examination of AFB1 biotransformation in consortium HS-1 will help us to explore these crucial bioresources more sensibly and efficiently.