背景:乳腺癌在全球范围内对女性民众来说是一个巨大的危险。乳腺癌与各种因素之间的关系,包括病毒感染,已被广泛调查。最近,HBV感染与乳腺癌患者之间的联系引起了人们的关注.本研究旨在评估在阿瓦士市诊断患有乳腺癌的妇女中HBV标志物的患病率,伊朗。
方法:从90例临床诊断为乳腺癌的患者中获取血清标本。患者的年龄从29岁到80岁不等,平均年龄49.42±10.7。活检标本的组织学检查显示75例(83.33%)为导管,11(8.88%)小叶,2(2.22%)粘液性,1(1.11%)髓质,转移性1例(1.11%)。通过ELISA对血清样品进行初始HBsAg和抗-HBc测试。随后通过巢式PCR和DNA测序分析测试血清阳性(HBsAg+抗-HBc)的样品的HBVS区。最后,构建了一个系统发育树,用于阳性HBVDNA测试。
结果:在5/90(5.55%)癌症患者中,发现3例(3.33%)导管癌和1例(1.11%)小叶癌显示HBV标志物阳性(HBsAg,抗HBc,HBVPCR)。值得注意的是,1例(1.11%)导管癌患者仅表现出抗HBc阳性。S区的系统发育树分析显示,所有确定的HBV菌株被归类为基因型D。
结论:统计分析没有发现任何显着发现(p=0.315)在不同年龄段的癌症类型的分布。在被诊断为乳腺癌的患者中,在HBV标志物中观察到5.5%的显著患病率.乳腺癌患者中的优势HBV基因型被鉴定为基因型D。
Breast cancer represents a formidable peril to the female populace on a worldwide level. The association between breast cancer and various factors, including viral infections, has been extensively investigated. Recently, the link between HBV infection and breast cancer patients has garnered attention. The present research aims to assess the prevalence of HBV markers among women diagnosed with breast cancer in Ahvaz city, Iran.
Serum specimens were procured from 90 patients who had been clinically diagnosed with breast cancer. The age of the patients ranged from 29 to 80 years, with a mean age of 49.42±10.7. Histological examination of biopsy specimens revealed that 75 (83.33%) were
ductal, 11 (8.88%) lobular, 2 (2.22%) mucinous, 1 (1.11%) medullary, and 1 (1.11%) was metastatic. The serum samples were subjected to initial HBsAg and anti-HBc testing via ELISA. Samples that tested seropositive (HBsAg + anti-HBc) were subsequently analyzed for the S region of HBV through nested PCR and DNA sequencing. Finally, a phylogenetic tree was constructed for positive HBV DNA tests.
Among the 5/90 (5.55%) cancer patients, it was found that 3 (3.33%) cases of
ductal carcinoma and one (1.11%) lobular carcinoma displayed positivity for HBV markers (HBsAg, anti-HBc, HBV PCR). Notably, one (1.11%) patient with
ductal carcinoma solely demonstrated anti-HBc positivity. The phylogenetic tree analysis of the S region revealed that all HBV strains identified were categorized as genotype D.
The statistical analysis did not reveal any significant findings (p= 0.315) in the distribution of cancer types across different age groups. Among patients diagnosed with breast cancer, a notable prevalence of 5.5% was observed in HBV markers. The dominant HBV genotype among breast cancer patients was identified as genotype D.